Platelet function in baboons and humans - A comparative study of whole blood using impedance platelet aggregometry (Multiplate®)

Martin Ponschab, Martijn van Griensven, Stefan Heitmeier, Volker Laux, Christoph J Schlimp, Andreas Calatzis, Soheyl Bahrami, Heinz Redl, Herbert Schöchl*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


BACKGROUND: Platelets play a pivotal role in coagulation, inflammation and wound healing. Suitable animal models that have the potential to mimic human platelet function are limited. The objective of the current study was to compare platelet aggregation response in the whole blood of baboons and humans using impedance aggregometry.

METHODS: Blood was drawn from 24 anesthetised male baboons and 25 healthy volunteers. The platelet aggregation response was determined by impedance aggregometry (Multiplate®). Platelets in the hirudinised whole blood samples were stimulated with four different activators: adenosine diphosphate (ADP), collagen (COL), thrombin receptor activating peptide-6 (TR1AP), and activation of PAR-4 thrombin receptor subtype (TR4AP) at standard concentrations. Higher than standard concentrations were tested in a subgroup of the animals.

RESULTS: The cell counts showed no differences between baboons and humans. The platelet aggregation response was significantly lower in baboons compared to humans when stimulated with the platelet agonists ADP (p<0.0001), COL (p=0.021) and TR4AP (p<0.0001). TR1AP did not stimulate platelet aggregation in the baboon blood. Doubling the concentration of ADP and of TR4AP significantly increased the AUC compared to the standard concentration. In contrast, increased COL levels did not further increase the AUC.

CONCLUSION: The current study revealed that testing the platelet function in baboon blood by impedance aggregometry is feasible with ADP, COL and TR4AP, but not with TR1AP. Compared to humans, the aggregation response is lower in baboons. Considering the limitations in accordance to these results, baboons might represent a potential species for further platelet research.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalThrombosis Research
Publication statusPublished - Nov 2016
Externally publishedYes


  • Adenosine Diphosphate/metabolism
  • Animals
  • Blood Platelets/cytology
  • Collagen/metabolism
  • Electric Impedance
  • Humans
  • Indicators and Reagents
  • Male
  • Papio
  • Peptide Fragments/metabolism
  • Platelet Aggregation
  • Platelet Function Tests/methods
  • Receptor, PAR-1/metabolism
  • Receptors, Thrombin/metabolism
  • Species Specificity
  • Platelet aggregometry
  • Coagulation
  • Baboons
  • ROTEM(R)
  • Platelets


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