TY - JOUR
T1 - Platelet Aggregation in Healthy Participants is Not Affected by Smoking, Drinking Coffee, Consuming a High-Fat Meal, or Performing Physical Exercise
AU - Krekels, Joyce P. M.
AU - Verhezen, Paul W. M.
AU - Henskens, Yvonne M. C.
N1 - Funding Information:
The authors thank the Hemostasis Working Group of the Dutch Society for Haematological Laboratories for critically reviewing this research project. The author(s) received no financial support for the research, authorship, and/or publication of this article.
Publisher Copyright:
© The Author(s) 2018.
PY - 2019/3/7
Y1 - 2019/3/7
N2 - Platelet aggregation can be measured using optical aggregation (light transmission aggregometry, LTA) as well as by impedance (Multiplate analyzer). The LTA (the gold standard method) can be influenced by many preanalytical variables. Several guidelines differ in recommendations for the duration patients should refrain from smoking, coffee, fatty meals, and physical exercise prior to blood collection for performing platelet function tests. In this pilot study, the influence of smoking, coffee, high-fat meal, or physical exercise on platelet aggregation was investigated to improve patient friendliness and laboratory logistics in platelet function diagnostics. Standardized blood collection was performed when participants were fasting and after each parameter (n=5 per group). As a control for diurnal fluctuations, participants (n=6) were fasting during both blood collections. Platelet aggregation was executed using standardized methods for LTA and Multiplate analyzer. Statistical analysis of the results using Wilcoxon signed-rank test did not show any significant differences in platelet aggregation in healthy participants under different preanalytical variables. Therefore, these variables are not expected to adversely affect testing, which can avoid canceling tests for those patients who inevitably did.
AB - Platelet aggregation can be measured using optical aggregation (light transmission aggregometry, LTA) as well as by impedance (Multiplate analyzer). The LTA (the gold standard method) can be influenced by many preanalytical variables. Several guidelines differ in recommendations for the duration patients should refrain from smoking, coffee, fatty meals, and physical exercise prior to blood collection for performing platelet function tests. In this pilot study, the influence of smoking, coffee, high-fat meal, or physical exercise on platelet aggregation was investigated to improve patient friendliness and laboratory logistics in platelet function diagnostics. Standardized blood collection was performed when participants were fasting and after each parameter (n=5 per group). As a control for diurnal fluctuations, participants (n=6) were fasting during both blood collections. Platelet aggregation was executed using standardized methods for LTA and Multiplate analyzer. Statistical analysis of the results using Wilcoxon signed-rank test did not show any significant differences in platelet aggregation in healthy participants under different preanalytical variables. Therefore, these variables are not expected to adversely affect testing, which can avoid canceling tests for those patients who inevitably did.
KW - clinical laboratory techniques
KW - electric impedance
KW - light
KW - platelet aggregation
KW - platelet function tests
KW - LIGHT TRANSMISSION AGGREGOMETRY
KW - CONJUGATED LINOLEIC-ACID
KW - PHYSIOLOGY SUBCOMMITTEE
KW - COAGULATION
KW - INHIBITION
KW - VARIABLES
KW - DIET
U2 - 10.1177/1076029618782445
DO - 10.1177/1076029618782445
M3 - Article
C2 - 29916260
SN - 1076-0296
VL - 25
SP - 1
EP - 9
JO - Clinical and Applied Thrombosis-Hemostasis
JF - Clinical and Applied Thrombosis-Hemostasis
M1 - 1076029618782445
ER -