Plasma of pregnant and preeclamptic women activates monocytes in vitro

M. M. Faas; R. B. Donker; M. G. van Pampus; A. M. F. Huls; J. Salomons; P. de Vos; J. G. Aarnoudse

doi:10.1016/j.ajog.2007.12.013

Plasma of pregnant and preeclamptic women activates monocytes in vitro

M. M. Faas
, R. B. Donker
, M. G. van Pampus
, A. M. F. Huls
, J. Salomons
, P. de Vos
, J. G. Aarnoudse

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

OBJECTIVE: The objective of the study was to test the hypothesis that factors circulating in the plasma of pregnant women and women with preeclampsia activate monocytes.STUDY DESIGN: Blood samples were taken from patients with early-onset severe preeclampsia (n = 9), healthy pregnant women (n = 9), and healthy nonpregnant women (n = 9). A monocytic cell line was incubated with the plasma for 4, 16, and 24 hours. After the incubation, reactive oxygen species (ROS) production and intercellular adhesion molecule (ICAM)-1 expression (protein and messenger ribonucleic acid) were measured.RESULTS: Plasma of both pregnant women and women with preeclampsia, as compared with plasma from nonpregnant women, increased the mean channel brightness (MCB) of ROS after 4 hours of incubation, whereas only plasma of pregnant women increased the percentage of cells producing ROS (after 4 and 24 hours of incubation). Plasma of pregnant women and women with preeclampsia upregulated the percentage of ICAM-1-expressing cells after 4 hours and down-regulated the percentage of ICAM-1-expressing cells and MCB after 24 hours.CONCLUSION: Plasma of both pregnant women and women with preeclampsia activated monocytes in vitro.

Original language	English
Article number	84.e1
Number of pages	8
Journal	American Journal of Obstetrics and Gynecology
Volume	199
Issue number	1
DOIs	https://doi.org/10.1016/j.ajog.2007.12.013
Publication status	Published - Jul 2008
Externally published	Yes
Event	52nd Annual Scientific Meeting (SGS): Advancing Gynecological Surgery: Growth through Adaptability and Accessibility - Hilton Phoenix Tapatio Cliffs Resort, Phoenix, United States Duration: 22 Mar 2005 → 25 Mar 2005 https://www.sgsonline.org/52nd-annual-scientific-meeting

Keywords

Intercellular adhesion molecule
Monocytes
Plasma
Preeclampsia
Pregnant
Reactive oxygen species

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10.1016/j.ajog.2007.12.013

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title = "Plasma of pregnant and preeclamptic women activates monocytes in vitro",

abstract = "OBJECTIVE: The objective of the study was to test the hypothesis that factors circulating in the plasma of pregnant women and women with preeclampsia activate monocytes.STUDY DESIGN: Blood samples were taken from patients with early-onset severe preeclampsia (n = 9), healthy pregnant women (n = 9), and healthy nonpregnant women (n = 9). A monocytic cell line was incubated with the plasma for 4, 16, and 24 hours. After the incubation, reactive oxygen species (ROS) production and intercellular adhesion molecule (ICAM)-1 expression (protein and messenger ribonucleic acid) were measured.RESULTS: Plasma of both pregnant women and women with preeclampsia, as compared with plasma from nonpregnant women, increased the mean channel brightness (MCB) of ROS after 4 hours of incubation, whereas only plasma of pregnant women increased the percentage of cells producing ROS (after 4 and 24 hours of incubation). Plasma of pregnant women and women with preeclampsia upregulated the percentage of ICAM-1-expressing cells after 4 hours and down-regulated the percentage of ICAM-1-expressing cells and MCB after 24 hours.CONCLUSION: Plasma of both pregnant women and women with preeclampsia activated monocytes in vitro.",

keywords = "Intercellular adhesion molecule, Monocytes, Plasma, Preeclampsia, Pregnant, Reactive oxygen species",

author = "Faas, \{M. M.\} and Donker, \{R. B.\} and \{van Pampus\}, \{M. G.\} and Huls, \{A. M. F.\} and J. Salomons and \{de Vos\}, P. and Aarnoudse, \{J. G.\}",

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AU - Donker, R. B.

AU - van Pampus, M. G.

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AU - Salomons, J.

AU - de Vos, P.

AU - Aarnoudse, J. G.

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Plasma of pregnant and preeclamptic women activates monocytes in vitro

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Keywords

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