Plasma Intestinal Fatty Acid-Binding Protein Levels Correlate With Morphologic Epithelial Intestinal Damage in a Human Translational Ischemia-reperfusion Model.

D.H. Schellekens, J. Grootjans, S.A. Dello, A.A. van Bijnen, R.M. van Dam, C.H. Dejong, J.P. Derikx, W.A. Buurman

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background and Aim:Intestinal fatty acid-binding protein (I-FABP) is a useful marker in the detection of intestinal ischemia. However, more insight into the test characteristics of I-FABP release is needed. This study aimed to investigate the relationship between plasma I-FABP levels and the severity of ischemic mucosal injury, and define the clinical usefulness of systemic I-FABP following ischemia.Methods:In a human experimental model, 6 cm of the jejunum, to be removed for surgical reasons, was selectively exposed to either 15, 30, or 60 minutes of ischemia (I) followed by 30 and 120 minutes of reperfusion (R). Blood and tissue was sampled at all time points. Arteriovenous (V-A) concentration differences of I-FABP were measured. Tissue sections were stained with hematoxylin/eosin, and villus height was measured to score epithelial damage.Results:Histologic analysis showed only minor reversible intestinal damage following 15I and 30I; however, severe irreversible epithelial damage was observed in the jejunum exposed to 60I. I-FABP V-A differences paralleled the degree of tissue damage over time [7.79 (1.8) ng/mL, 128.6 (+/- 44.2) ng/mL, 463.3 (+/- 139.8) ng/mL for 15I, 30I and 60I, respectively]. A good correlation was found between histologic epithelial damage and V-A I-FABP (r=-0.82, P

Original languageEnglish
Pages (from-to)253-260
Number of pages8
JournalJournal of Clinical Gastroenterology
Volume48
Issue number3
DOIs
Publication statusPublished - Mar 2014

Keywords

  • intestinal ischemia
  • acute abdomen
  • enterocyte damage
  • biomarker
  • human
  • diagnostic characteristics
  • ACUTE MESENTERIC ISCHEMIA
  • AORTIC-SURGERY
  • EARLY MARKER
  • INJURY
  • INFLAMMATION
  • ACCURACY
  • BARRIER
  • BOWEL
  • FABP

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