Plasma FFA utilization and FABP content are diminished in forearm skeletal muscle of type 2 diabetic subjects

E.E. Blaak*, A.J.M. Wagenmakers, J.F. Glatz, B.H.R. Wolffenbuttel, G.J. Kemerink, C.J.M. Langenberg, G.A.K. Heidendal, W.H.M. Saris

*Corresponding author for this work

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In this study, we investigated the hypothesis that impairments in forearm skeletal muscle free fatty acid (FFA) metabolism are present in patients with type 2 diabetes both in the overnight fasted state and during beta-adrenergic stimulation. Eight obese subjects with type 2 diabetes and eight nonobese controls (Con) were studied using the forearm balance technique and indirect calorimetry during infusion of the stable isotope tracer [U-(13)C]palmitate after an overnight fast and during infusion of the nonselective beta-agonist isoprenaline (Iso, 20 ng. kg lean body mass(-1) x min(-1)). Additionally, activities of mitochondrial enzymes and of cytoplasmatic fatty acid-binding protein (FABP) were determined in biopsies from the vastus lateralis muscle. Both during fasting and Iso infusion, the tracer balance data showed that forearm muscle FFA uptake (Con vs. type 2: fast 449+/-69 vs. 258 +/-42 and Iso 715+/-129 vs. 398+/-70 nmol. 100 ml tissue(-1) x min(-1), P
Original languageEnglish
Pages (from-to)E146-154
Number of pages8
JournalAmerican Journal of Physiology : Endocrinology and Metabolism
Issue number1
Publication statusPublished - 1 Jan 2000

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