TY - JOUR
T1 - Physical activity and markers of glycation in older individuals
T2 - data from a combined cross-sectional and randomized controlled trial (EXAMIN AGE)
AU - Van den Eynde, Mathias D. G.
AU - Streese, Lukas
AU - Houben, Alfons J. H. M.
AU - Stehouwer, Coen D. A.
AU - Scheijen, Jean L. J. M.
AU - Schalkwijk, Casper G.
AU - Hanssen, Nordin M. J.
AU - Hanssen, Henner
PY - 2020/5
Y1 - 2020/5
N2 - Background: Advanced glycation end products (AGEs) are protein modifications that are predominantly formed from dicarbonyl compounds that arise from glucose and lipid metabolism. AGEs and sedentary behavior have been identified as a driver of accelerated (vascular) aging. The effect of physical activity on AGE accumulation is unknown. Therefore, we investigated whether plasma AGEs and dicarbonyl levels are different across older individuals that were active or sedentary and whether plasma AGEs are affected by high-intensity interval training (HIIT).Methods: We included healthy older active (HA, n=38, 44.7% female, 60.1 +/- 7.7 years old) and healthy older sedentary (HS, n=36, 72.2% female, 60.0 +/- 7.3 years old) individuals as well as older sedentary individuals with increased cardiovascular risk (SR, n=84, 50% female, 58.7 +/- 6.6 years old). The SR group was randomized into a 12-week walking-based HIIT program or control group. We measured protein-bound and free plasma AGEs and dicarbonyls by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) at baseline and after the HIIT intervention.Results: Protein-bound AGE Ne-(carboxymethyl)lysine (CML) was lower in SR (2.6 +/- 0.5 mu mol/l) and HS (3.1 +/- 0.5 mu mol/l) than in HA (3.6 +/- 0.6 mu mol/l; PDiscussion: Although lifestyle interventions may act as important modulators of cardiovascular risk, HIIT is not a potent short-term intervention to reduce glycation in older individuals, underlining the need for other approaches, such as pharmacological agents, to reduce AGEs and lower cardiovascular risk in this population.
AB - Background: Advanced glycation end products (AGEs) are protein modifications that are predominantly formed from dicarbonyl compounds that arise from glucose and lipid metabolism. AGEs and sedentary behavior have been identified as a driver of accelerated (vascular) aging. The effect of physical activity on AGE accumulation is unknown. Therefore, we investigated whether plasma AGEs and dicarbonyl levels are different across older individuals that were active or sedentary and whether plasma AGEs are affected by high-intensity interval training (HIIT).Methods: We included healthy older active (HA, n=38, 44.7% female, 60.1 +/- 7.7 years old) and healthy older sedentary (HS, n=36, 72.2% female, 60.0 +/- 7.3 years old) individuals as well as older sedentary individuals with increased cardiovascular risk (SR, n=84, 50% female, 58.7 +/- 6.6 years old). The SR group was randomized into a 12-week walking-based HIIT program or control group. We measured protein-bound and free plasma AGEs and dicarbonyls by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) at baseline and after the HIIT intervention.Results: Protein-bound AGE Ne-(carboxymethyl)lysine (CML) was lower in SR (2.6 +/- 0.5 mu mol/l) and HS (3.1 +/- 0.5 mu mol/l) than in HA (3.6 +/- 0.6 mu mol/l; PDiscussion: Although lifestyle interventions may act as important modulators of cardiovascular risk, HIIT is not a potent short-term intervention to reduce glycation in older individuals, underlining the need for other approaches, such as pharmacological agents, to reduce AGEs and lower cardiovascular risk in this population.
KW - DICARBONYL STRESS
KW - END-PRODUCTS
KW - CARDIOVASCULAR EVENTS
KW - VASCULAR FUNCTION
KW - OXIDATIVE STRESS
KW - EXERCISE
KW - MECHANISMS
KW - INFLAMMATION
KW - ENDPRODUCTS
KW - OVERWEIGHT
U2 - 10.1042/CS20200255
DO - 10.1042/CS20200255
M3 - Article
C2 - 32356559
VL - 134
SP - 1095
EP - 1105
JO - Clinical Science
JF - Clinical Science
SN - 0143-5221
IS - 9
ER -