TY - JOUR
T1 - Photo/thermo-sensitive chitosan and gelatin-based interpenetrating polymer network for mimicking muscle tissue extracellular matrix
AU - Stanzione, Antonella
AU - Polini, Alessandro
AU - Scalera, Francesca
AU - Gigli, Giuseppe
AU - Moroni, Lorenzo
AU - Gervaso, Francesca
N1 - Funding Information:
The authors are grateful to the \u201CTecnopolo per la medicina di precisione\u201D (TecnoMed Puglia) - Regione Puglia: DGR n.2117 del 21/11/ 2018, CUP: B84I18000540002 and to the Italian Ministry of Research (MUR) under the EU NRRP \u201CNational Center for Gene Therapy and Drugs based on RNA Technology\u201D (Project no. CN00000041 CN3 RNA) and the complementary actions to the EU NRRP \u201CFIT4MedRob\u201D Grant (contract number CUP B53C22006960001) and \u201CD34 Health\u201D (contract number, CUP B53C22006100001). The Project PON-SHINE (POTENZIAMENTO DEI NODI ITALIANI IN E-RIHS, CIG: Z902D25DCE \u2013 CUP: B27E19000030007) funded by Italian MIUR is acknowledged. A. P. and F. G. gratefully acknowledge the support from the European Union\u2019s Horizon 2020 research and innovation programme under grant agreement No. 953121 (FLAMIN-GO).
Funding Information:
The authors are grateful to the \u201CTecnopolo per la medicina di precisione\u201D (TecnoMed Puglia) - Regione Puglia: DGR n.2117 del 21/11/2018, CUP: B84I18000540002 and to the Italian Ministry of University and Research (MUR) under the EU NRRP \u201CNational Center for Gene Therapy and Drugs based on RNA Technology\u201D (Project no. CN00000041 CN3 RNA) and the complementary actions to the EU NRRP \u201CFIT4MedRob\u201D Grant (contract number CUP B53C22006960001) and \u201CD34 Health\u201D (contract number, CUP B53C22006100001). The Project PON-SHINE (POTENZIAMENTO DEI NODI ITALIANI IN E-RIHS, CIG: Z902D25DCE \u2013 CUP: B27E19000030007) funded by Italian MIUR is acknowledged. A. P. and F. G. gratefully acknowledge the support from the European Union's Horizon 2020 research and innovation programme under grant agreement No. 953121 (FLAMIN-GO).
Publisher Copyright:
© 2024 The Author(s)
PY - 2024/11/15
Y1 - 2024/11/15
N2 - The dynamic interplay between extracellular matrix (ECM), its 3D architecture and resident cells plays a pivotal role in cell behavior influencing essential processes like proliferation, migration, and differentiation. Matrix-based 3D culture systems have emerged as valuable tools to model organ and tissue interactions in vitro. A 3D matrix analog must possess high biocompatibility and fully reproduce the characteristics of the native tissue in terms of mechanical properties. In this regard, interpenetrating polymer networks (IPNs) are particularly attractive because of the high tunability of their physicochemical properties. In this study, a chitosan (Ch) and modified gelatin (GelMA) IPN with a sol-gel transition triggered by two external physical stimuli, UV light and temperature, was designed to mimic the muscle tissue ECM in terms of mechanical stiffness. The system was deeply characterized demonstrating to support not only the growth and viability of muscle cells embedded within the hydrogel, but also cell differentiation toward muscle phenotype.
AB - The dynamic interplay between extracellular matrix (ECM), its 3D architecture and resident cells plays a pivotal role in cell behavior influencing essential processes like proliferation, migration, and differentiation. Matrix-based 3D culture systems have emerged as valuable tools to model organ and tissue interactions in vitro. A 3D matrix analog must possess high biocompatibility and fully reproduce the characteristics of the native tissue in terms of mechanical properties. In this regard, interpenetrating polymer networks (IPNs) are particularly attractive because of the high tunability of their physicochemical properties. In this study, a chitosan (Ch) and modified gelatin (GelMA) IPN with a sol-gel transition triggered by two external physical stimuli, UV light and temperature, was designed to mimic the muscle tissue ECM in terms of mechanical stiffness. The system was deeply characterized demonstrating to support not only the growth and viability of muscle cells embedded within the hydrogel, but also cell differentiation toward muscle phenotype.
U2 - 10.1016/j.heliyon.2024.e39820
DO - 10.1016/j.heliyon.2024.e39820
M3 - Article
SN - 2405-8440
VL - 10
JO - Heliyon
JF - Heliyon
IS - 21
M1 - e39820
ER -