Phosphatidylinositol 4-kinase III beta mediates contraction-induced GLUT4 translocation and shows its anti-diabetic action in cardiomyocytes

A. Sun, O. Simsek Papur, E. Dirkx, L. Wong, T. Sips, S. Wang, A. Strzelecka, M. Nabben, J. F. C. Glatz, D. Neumann, J. J. F. P. Luiken*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Web of Science)

Abstract

In the diabetic heart, long-chain fatty acid (LCFA) uptake is increased at the expense of glucose uptake. This metabolic shift ultimately leads to insulin resistance and a reduced cardiac function. Therefore, signaling kinases that mediate glucose uptake without simultaneously stimulating LCFA uptake could be considered attractive anti-diabetic targets. Phosphatidylinositol-4-kinase-III beta (PI4KIII beta) is a lipid kinase downstream of protein kinase D1 (PKD1) that mediates Golgi-to-plasma membrane vesicular trafficking in HeLa-cells. In this study, we evaluated whether PI4KIII beta is involved in myocellular GLUT4 translocation induced by contraction or oligomycin (an F1F0-ATP synthase inhibitor that activates contraction-like signaling). Pharmacological targeting, with compound MI14, or genetic silencing of PI4KIII beta inhibited contraction/oligomycin-stimulated GLUT4 translocation and glucose uptake in cardiomyocytes but did not affect CD36 translocation nor LCFA uptake. Addition of the PI4KIII beta enzymatic reaction product phosphatidylinositol-4-phosphate restored oligomycin-stimulated glucose uptake in the presence of MI14. PI4KIII beta activation by PKD1 involves Ser294 phosphorylation and altered its localization with unchanged enzymatic activity. Adenoviral PI4KIII beta overexpression stimulated glucose uptake, but did not activate hypertrophic signaling, indicating that unlike PKD1, PI4KIII beta is selectively involved in GLUT4 translocation. Finally, PI4KIII beta overexpression prevented insulin resistance and contractile dysfunction in lipid-overexposed cardiomyocytes. Together, our studies identify PI4KIII beta as positive and selective regulator of GLUT4 translocation in response to contraction-like signaling, suggesting PI4KIII beta as a promising target to rescue defective glucose uptake in diabetics.

Original languageEnglish
Pages (from-to)2839-2856
Number of pages18
JournalCellular and Molecular Life Sciences
Volume78
Issue number6
Early online date22 Oct 2020
DOIs
Publication statusPublished - Mar 2021

Keywords

  • Diabetic cardiomyopathy
  • Glucose transport
  • GLUT4 translocation
  • Insulin resistance
  • Phosphatidylinositol-4-kinase-III&#946
  • PROTEIN-KINASE-D
  • RAT CARDIAC MYOCYTES
  • FATTY-ACID UPTAKE
  • INSULIN-RESISTANCE
  • LIPID-ACCUMULATION
  • GLUCOSE-UPTAKE
  • TRANSPORT
  • MEMBRANE
  • AMINOPEPTIDASE
  • METABOLISM

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