Pharmacokinetic properties of the synthetic cannabinoid JWH-018 in oral fluid after inhalation

Stefan W. Toennes, Anna Geraths, Werner Pogoda, Alexander Paulke, Cora Wunder, Eef L Theunissen, Johannes G Ramaekers

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Each year, synthetic cannabinoids occur in high numbers on the illicit drug market, but data on their detectability are rarely available. A pilot study was performed to assess adverse effects of JWH-018, which is one of the oldest and best known synthetic cannabinoids. Oral fluid has been evaluated as a specimen for drug monitoring. Six subjects inhaled smoke derived from 2 and 3 mg JWH-018. The drug and 10 of its metabolites were analyzed in oral fluid samples collected during the following 12 hours using the Quantisal collection device by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Maximum concentrations of JWH-018 reached 2.2-2036 (median 25.7) ng/mL after inhalation and decreased during the next hour to only 0.08-8.42 (median 0.89) ng/mL. Metabolites were not found. During the elimination phase (median half-life 1.69 hours), detection of the drug over 6-12 hours (median 8 hours) after inhalation was achieved (0.024 ng/mL limit of quantification). Oral fluid/serum ratios varied considerably intra- and inter-individually in a range of 0.05-555 (median 1.38). The detection of JWH-018 in oral fluid requires high analytical sensitivity even 1 hour after inhalation. The pharmacokinetic properties of inhaled JWH-018 are similar to those of THC. Times for detection are typically less than 12 hours. High variability of the oral fluid/serum ratio precludes extrapolation of oral fluid concentrations to blood.

Original languageEnglish
Pages (from-to)644-650
Number of pages7
JournalDrug Testing and Analysis
Volume10
Issue number4
Early online date2 Oct 2017
DOIs
Publication statusPublished - Apr 2018

Keywords

  • pharmacokinetics
  • synthetic cannabinoids
  • JWH-018
  • oral fluid
  • liquid chromatography-mass spectrometry
  • TANDEM MASS-SPECTROMETRY
  • LC-MS-MS
  • DESIGNER DRUGS
  • SERUM
  • QUANTIFICATION
  • DELTA(9)-TETRAHYDROCANNABINOL
  • ABUSE
  • IDENTIFICATION
  • BLOOD
  • MS/MS
  • Chromatography, Liquid/methods
  • Humans
  • Male
  • Naphthalenes/administration & dosage
  • Indoles/administration & dosage
  • Young Adult
  • Substance Abuse Detection/methods
  • Adult
  • Female
  • Limit of Detection
  • Tandem Mass Spectrometry/methods
  • Saliva/metabolism
  • Administration, Inhalation
  • Cannabinoids/administration & dosage
  • Pilot Projects
  • Street Drugs/metabolism

Cite this

Toennes, Stefan W. ; Geraths, Anna ; Pogoda, Werner ; Paulke, Alexander ; Wunder, Cora ; Theunissen, Eef L ; Ramaekers, Johannes G. / Pharmacokinetic properties of the synthetic cannabinoid JWH-018 in oral fluid after inhalation. In: Drug Testing and Analysis. 2018 ; Vol. 10, No. 4. pp. 644-650.
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abstract = "Each year, synthetic cannabinoids occur in high numbers on the illicit drug market, but data on their detectability are rarely available. A pilot study was performed to assess adverse effects of JWH-018, which is one of the oldest and best known synthetic cannabinoids. Oral fluid has been evaluated as a specimen for drug monitoring. Six subjects inhaled smoke derived from 2 and 3 mg JWH-018. The drug and 10 of its metabolites were analyzed in oral fluid samples collected during the following 12 hours using the Quantisal collection device by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Maximum concentrations of JWH-018 reached 2.2-2036 (median 25.7) ng/mL after inhalation and decreased during the next hour to only 0.08-8.42 (median 0.89) ng/mL. Metabolites were not found. During the elimination phase (median half-life 1.69 hours), detection of the drug over 6-12 hours (median 8 hours) after inhalation was achieved (0.024 ng/mL limit of quantification). Oral fluid/serum ratios varied considerably intra- and inter-individually in a range of 0.05-555 (median 1.38). The detection of JWH-018 in oral fluid requires high analytical sensitivity even 1 hour after inhalation. The pharmacokinetic properties of inhaled JWH-018 are similar to those of THC. Times for detection are typically less than 12 hours. High variability of the oral fluid/serum ratio precludes extrapolation of oral fluid concentrations to blood.",
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Pharmacokinetic properties of the synthetic cannabinoid JWH-018 in oral fluid after inhalation. / Toennes, Stefan W.; Geraths, Anna; Pogoda, Werner; Paulke, Alexander; Wunder, Cora; Theunissen, Eef L; Ramaekers, Johannes G.

In: Drug Testing and Analysis, Vol. 10, No. 4, 04.2018, p. 644-650.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Toennes, Stefan W.

AU - Geraths, Anna

AU - Pogoda, Werner

AU - Paulke, Alexander

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AU - Theunissen, Eef L

AU - Ramaekers, Johannes G

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AB - Each year, synthetic cannabinoids occur in high numbers on the illicit drug market, but data on their detectability are rarely available. A pilot study was performed to assess adverse effects of JWH-018, which is one of the oldest and best known synthetic cannabinoids. Oral fluid has been evaluated as a specimen for drug monitoring. Six subjects inhaled smoke derived from 2 and 3 mg JWH-018. The drug and 10 of its metabolites were analyzed in oral fluid samples collected during the following 12 hours using the Quantisal collection device by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Maximum concentrations of JWH-018 reached 2.2-2036 (median 25.7) ng/mL after inhalation and decreased during the next hour to only 0.08-8.42 (median 0.89) ng/mL. Metabolites were not found. During the elimination phase (median half-life 1.69 hours), detection of the drug over 6-12 hours (median 8 hours) after inhalation was achieved (0.024 ng/mL limit of quantification). Oral fluid/serum ratios varied considerably intra- and inter-individually in a range of 0.05-555 (median 1.38). The detection of JWH-018 in oral fluid requires high analytical sensitivity even 1 hour after inhalation. The pharmacokinetic properties of inhaled JWH-018 are similar to those of THC. Times for detection are typically less than 12 hours. High variability of the oral fluid/serum ratio precludes extrapolation of oral fluid concentrations to blood.

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KW - BLOOD

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KW - Female

KW - Limit of Detection

KW - Tandem Mass Spectrometry/methods

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KW - Administration, Inhalation

KW - Cannabinoids/administration & dosage

KW - Pilot Projects

KW - Street Drugs/metabolism

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