Performance of severity parameters to detect chemotherapy-induced pain and distress in mice

Anne Rix, Natascha Drude, Anna Mrugalla, Felix M. Mottaghy, Rene H. Tolba*, Fabian Kiessling

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Web of Science)

Abstract

According to European Union directive 2010/63/EU a severity classification of experimental procedures performed on laboratory animals is mandatory. This includes a prospective evaluation of all interventions performed within the experiment, as well as an assessment of the actual burden of each animal during the experiment. In this regard, the evaluation and scoring of defined criteria regarding the health state of animals could help to early identify deteriorations in animal health and facilitate the application of humane endpoints. This article discusses the applicability of an adapted score sheet in BALB/cAnNRj mice receiving either cisplatin, doxorubicin or busulfan, three chemotherapeutic agents with different toxicological profiles and longitudinal non-invasive molecular imaging. The health state was investigated by score sheets documenting general state, body weight, spontaneous behaviour and treatment specific parameters (e.g. anaemia, neurotoxicity, persistent diarrhoea). Although blood and serum analyses clearly indicated various organ damage, most scoring parameters except for body weight did not report on the deceasing animal health state. Thus, there is need for more sensitive observational parameters to judge the animal's health state and welfare.

Original languageEnglish
Article number0023677219883327
Pages (from-to)452-460
Number of pages9
JournalLaboratory Animals
Volume54
Issue number5
Early online date29 Oct 2019
DOIs
Publication statusPublished - Oct 2020

Keywords

  • Severity assessment
  • score sheet
  • chemotherapy
  • imaging
  • MOUSE MODEL
  • WORKING GROUP
  • DOXORUBICIN
  • ANIMALS
  • BEHAVIOR

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