Performance in Omics Analyses of Blood Samples in Long-Term Storage: Opportunities for the Exploitation of Existing Biobanks in Environmental Health Research

Dennie G. A. J. Hebels; Panagiotis Georgiadis; Hector C. Keun; Toby J. Athersuch; Paolo Vineis; Roel Vermeulen; Lutzen Portengen; Ingvar A. Bergdahl; Goran Hallmans; Domenico Palli; Benedetta Bendinelli; Vittorio Krogh; Rosario Tumino; Carlotta Sacerdote; Salvatore Panico; Jos C. S. Kleinjans; Theo M. C. M. de Kok; Martyn T. Smith; Soterios A. Kyrtopoulos

doi:10.1289/ehp.1205657

Performance in Omics Analyses of Blood Samples in Long-Term Storage: Opportunities for the Exploitation of Existing Biobanks in Environmental Health Research

Dennie G. A. J. Hebels
, Panagiotis Georgiadis
, Hector C. Keun
, Toby J. Athersuch
, Paolo Vineis
, Roel Vermeulen
, Lutzen Portengen
, Ingvar A. Bergdahl
, Goran Hallmans
, Domenico Palli
, Benedetta Bendinelli
, Vittorio Krogh
, Rosario Tumino
, Carlotta Sacerdote
, Salvatore Panico
, Jos C. S. Kleinjans
, Theo M. C. M. de Kok
, Martyn T. Smith
, Soterios A. Kyrtopoulos^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown. OBJECTIVES: We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [ UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles. METHODS: We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for = 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of time-in-storage was observed in samples stored over a period of 13-17 years. CONCLUSIONS: Most samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.

Original language	English
Pages (from-to)	480-487
Number of pages	8
Journal	Environmental Health Perspectives
Volume	121
Issue number	4
DOIs	https://doi.org/10.1289/ehp.1205657
Publication status	Published - Apr 2013

Keywords

biomarkers
epigenomics
metabolomics
metabonomics
molecular epidemiology
proteomics
transcriptomics

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Hebels, D. G. A. J., Georgiadis, P., Keun, H. C., Athersuch, T. J., Vineis, P., Vermeulen, R., Portengen, L., Bergdahl, I. A., Hallmans, G., Palli, D., Bendinelli, B., Krogh, V., Tumino, R., Sacerdote, C., Panico, S., Kleinjans, J. C. S., de Kok, T. M. C. M., Smith, M. T., & Kyrtopoulos, S. A. (2013). Performance in Omics Analyses of Blood Samples in Long-Term Storage: Opportunities for the Exploitation of Existing Biobanks in Environmental Health Research. Environmental Health Perspectives, 121(4), 480-487. https://doi.org/10.1289/ehp.1205657

@article{5d1dbaf8357e491887c7b44a1853f60d,

title = "Performance in Omics Analyses of Blood Samples in Long-Term Storage: Opportunities for the Exploitation of Existing Biobanks in Environmental Health Research",

abstract = "BACKGROUND: The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown. OBJECTIVES: We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [ UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles. METHODS: We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for = 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of time-in-storage was observed in samples stored over a period of 13-17 years. CONCLUSIONS: Most samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.",

keywords = "biomarkers, epigenomics, metabolomics, metabonomics, molecular epidemiology, proteomics, transcriptomics",

author = "Hebels, \{Dennie G. A. J.\} and Panagiotis Georgiadis and Keun, \{Hector C.\} and Athersuch, \{Toby J.\} and Paolo Vineis and Roel Vermeulen and Lutzen Portengen and Bergdahl, \{Ingvar A.\} and Goran Hallmans and Domenico Palli and Benedetta Bendinelli and Vittorio Krogh and Rosario Tumino and Carlotta Sacerdote and Salvatore Panico and Kleinjans, \{Jos C. S.\} and \{de Kok\}, \{Theo M. C. M.\} and Smith, \{Martyn T.\} and Kyrtopoulos, \{Soterios A.\}",

year = "2013",

month = apr,

doi = "10.1289/ehp.1205657",

language = "English",

volume = "121",

pages = "480--487",

journal = "Environmental Health Perspectives",

issn = "0091-6765",

publisher = "Public Health Services, US Dept of Health and Human Services",

number = "4",

}

Hebels, DGAJ, Georgiadis, P, Keun, HC, Athersuch, TJ, Vineis, P, Vermeulen, R, Portengen, L, Bergdahl, IA, Hallmans, G, Palli, D, Bendinelli, B, Krogh, V, Tumino, R, Sacerdote, C, Panico, S, Kleinjans, JCS, de Kok, TMCM, Smith, MT & Kyrtopoulos, SA 2013, 'Performance in Omics Analyses of Blood Samples in Long-Term Storage: Opportunities for the Exploitation of Existing Biobanks in Environmental Health Research', Environmental Health Perspectives, vol. 121, no. 4, pp. 480-487. https://doi.org/10.1289/ehp.1205657

Performance in Omics Analyses of Blood Samples in Long-Term Storage: Opportunities for the Exploitation of Existing Biobanks in Environmental Health Research. / Hebels, Dennie G. A. J.; Georgiadis, Panagiotis; Keun, Hector C. et al.
In: Environmental Health Perspectives, Vol. 121, No. 4, 04.2013, p. 480-487.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Performance in Omics Analyses of Blood Samples in Long-Term Storage: Opportunities for the Exploitation of Existing Biobanks in Environmental Health Research

AU - Hebels, Dennie G. A. J.

AU - Georgiadis, Panagiotis

AU - Keun, Hector C.

AU - Athersuch, Toby J.

AU - Vineis, Paolo

AU - Vermeulen, Roel

AU - Portengen, Lutzen

AU - Bergdahl, Ingvar A.

AU - Hallmans, Goran

AU - Palli, Domenico

AU - Bendinelli, Benedetta

AU - Krogh, Vittorio

AU - Tumino, Rosario

AU - Sacerdote, Carlotta

AU - Panico, Salvatore

AU - Kleinjans, Jos C. S.

AU - de Kok, Theo M. C. M.

AU - Smith, Martyn T.

AU - Kyrtopoulos, Soterios A.

PY - 2013/4

Y1 - 2013/4

N2 - BACKGROUND: The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown. OBJECTIVES: We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [ UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles. METHODS: We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for = 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of time-in-storage was observed in samples stored over a period of 13-17 years. CONCLUSIONS: Most samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.

AB - BACKGROUND: The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown. OBJECTIVES: We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [ UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles. METHODS: We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for = 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of time-in-storage was observed in samples stored over a period of 13-17 years. CONCLUSIONS: Most samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.

KW - biomarkers

KW - epigenomics

KW - metabolomics

KW - metabonomics

KW - molecular epidemiology

KW - proteomics

KW - transcriptomics

U2 - 10.1289/ehp.1205657

DO - 10.1289/ehp.1205657

M3 - Article

C2 - 23384616

SN - 0091-6765

VL - 121

SP - 480

EP - 487

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

IS - 4

ER -

Performance in Omics Analyses of Blood Samples in Long-Term Storage: Opportunities for the Exploitation of Existing Biobanks in Environmental Health Research

Abstract

Keywords

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