Passive smoking alters circulating naive/memory lymphocyte T-cell subpopulations in children

C.I. Vardavas, M. Plada, M. Tzatzarakis, A. Marcos, J. Warnberg, S. Gomez Martinez, C. Breidenassel, M. Gonzalez Gross, A.M. Tsatsakis, W.H.M. Saris, L.A. Moreno, A.G. Kafatos

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

While it has been indicated that exposure to second-hand smoke (SHS) can cause a local in vivo response, limited evidence exists on its possible systemic effects from population-based levels of exposure. We investigated into a possible systemic response in the immune parameters and lymphocyte subsets, i.e. B cell (CD19+), T cell (CD4+CD45RO+, CD4+CD45RA+, CD3+CD45RO+, CD3+CD45RA+) and natural killer (CD3+CD16CD56+) lymphocyte subsets relative to exposure to SHS. Blood was drawn from healthy, verified non-smoker, adolescent subjects (n = 68, mean age 14.2) and analysed for cotinine, antioxidants and lymphocyte immunophenotyping. SHS exposure was assessed using serum cotinine. Biomarker quantified exposure to SHS was correlated with a linear dose-response reduction in the percentages of memory CD4+CD45RO+ (p = 0.005) and CD3+CD45RO+ T-cell subsets (p = 0.005 and p = 0.003, respectively) and a linear increase in the percentage of naive CD4+CD45RA+ and CD3+CD45RA+ T-cell subsets (p = 0.006 and p = 0.003, respectively). Additionally, higher exposure to SHS was associated with a higher CD4+CD45RA+ count (532 vs. 409 cells/ml, p = 0.017). Moreover, after controlling for age, gender, body mass index and plasma antioxidants, SHS exposure was found to be associated with the percentage of circulating naive and memory CD4+ and CD3+ T-cell subpopulations, as revealed through a linear regression analysis. These findings indicate a systemic immunological response in healthy adolescents exposed to population-based levels of SHS exposure and imply an additional biological pathway for the interaction between exposure to SHS and its adverse effects on human health.

Original languageEnglish
Pages (from-to)1171-1178
Number of pages8
JournalPediatric Allergy and Immunology
Volume21
Issue number8
DOIs
Publication statusPublished - Dec 2010

Keywords

  • passive smoking
  • immune response
  • children
  • lymphocytes
  • second-hand smoke
  • ENVIRONMENTAL TOBACCO-SMOKE
  • EXPOSURE
  • BIOMARKER
  • STABILITY
  • COTININE

Cite this

Vardavas, C. I., Plada, M., Tzatzarakis, M., Marcos, A., Warnberg, J., Gomez Martinez, S., Breidenassel, C., Gonzalez Gross, M., Tsatsakis, A. M., Saris, W. H. M., Moreno, L. A., & Kafatos, A. G. (2010). Passive smoking alters circulating naive/memory lymphocyte T-cell subpopulations in children. Pediatric Allergy and Immunology, 21(8), 1171-1178. https://doi.org/10.1111/j.1399-3038.2010.01039.x