Oxygen-Dependent Lipid Profiles of Three-Dimensional Cultured Human Chondrocytes Revealed by MALDI-MSI

Brenda Bakker; Gert B. Eijkel; Ron M. A. Heeren; Marcel Karperien; Janine N. Post; Berta Cillero-Pastor

doi:10.1021/acs.analchem.7b02265

Oxygen-Dependent Lipid Profiles of Three-Dimensional Cultured Human Chondrocytes Revealed by MALDI-MSI

Brenda Bakker
, Gert B. Eijkel
, Ron M. A. Heeren
, Marcel Karperien
, Janine N. Post
, Berta Cillero-Pastor^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Articular cartilage is exposed to a gradient of oxygen levels ranging from 5% at the surface to 1% in the deepest layers. While most cartilage research is performed in supra physiological oxygen levels (19-21%), culturing chondrocytes under hypoxic oxygen levels (

Original language	English
Pages (from-to)	9438-9444
Number of pages	7
Journal	Analytical Chemistry
Volume	89
Issue number	17
DOIs	https://doi.org/10.1021/acs.analchem.7b02265
Publication status	Published - 5 Sept 2017

Keywords

ION MASS-SPECTROMETRY
MITOCHONDRIAL CARDIOLIPIN
HYPOXIA
CARTILAGE
DIFFERENTIATION
METABOLISM
TENSION
PATHOPHYSIOLOGY
CHONDROGENESIS
OSTEOARTHRITIS

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title = "Oxygen-Dependent Lipid Profiles of Three-Dimensional Cultured Human Chondrocytes Revealed by MALDI-MSI",

abstract = "Articular cartilage is exposed to a gradient of oxygen levels ranging from 5\% at the surface to 1\% in the deepest layers. While most cartilage research is performed in supra physiological oxygen levels (19-21\%), culturing chondrocytes under hypoxic oxygen levels (",

keywords = "ION MASS-SPECTROMETRY, MITOCHONDRIAL CARDIOLIPIN, HYPOXIA, CARTILAGE, DIFFERENTIATION, METABOLISM, TENSION, PATHOPHYSIOLOGY, CHONDROGENESIS, OSTEOARTHRITIS",

author = "Brenda Bakker and Eijkel, \{Gert B.\} and Heeren, \{Ron M. A.\} and Marcel Karperien and Post, \{Janine N.\} and Berta Cillero-Pastor",

year = "2017",

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doi = "10.1021/acs.analchem.7b02265",

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journal = "Analytical Chemistry",

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T1 - Oxygen-Dependent Lipid Profiles of Three-Dimensional Cultured Human Chondrocytes Revealed by MALDI-MSI

AU - Bakker, Brenda

AU - Eijkel, Gert B.

AU - Heeren, Ron M. A.

AU - Karperien, Marcel

AU - Post, Janine N.

AU - Cillero-Pastor, Berta

PY - 2017/9/5

Y1 - 2017/9/5

N2 - Articular cartilage is exposed to a gradient of oxygen levels ranging from 5% at the surface to 1% in the deepest layers. While most cartilage research is performed in supra physiological oxygen levels (19-21%), culturing chondrocytes under hypoxic oxygen levels (

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KW - ION MASS-SPECTROMETRY

KW - MITOCHONDRIAL CARDIOLIPIN

KW - HYPOXIA

KW - CARTILAGE

KW - DIFFERENTIATION

KW - METABOLISM

KW - TENSION

KW - PATHOPHYSIOLOGY

KW - CHONDROGENESIS

KW - OSTEOARTHRITIS

U2 - 10.1021/acs.analchem.7b02265

DO - 10.1021/acs.analchem.7b02265

M3 - Article

C2 - 28727417

SN - 0003-2700

VL - 89

SP - 9438

EP - 9444

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 17

ER -

Oxygen-Dependent Lipid Profiles of Three-Dimensional Cultured Human Chondrocytes Revealed by MALDI-MSI

Abstract

Keywords

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