Oxygen-Dependent Lipid Profiles of Three-Dimensional Cultured Human Chondrocytes Revealed by MALDI-MSI

Brenda Bakker; Gert B. Eijkel; Ron M. A. Heeren; Marcel Karperien; Janine N. Post; Berta Cillero-Pastor

doi:10.1021/acs.analchem.7b02265

Oxygen-Dependent Lipid Profiles of Three-Dimensional Cultured Human Chondrocytes Revealed by MALDI-MSI

Brenda Bakker, Gert B. Eijkel, Ron M. A. Heeren, Marcel Karperien, Janine N. Post, Berta Cillero-Pastor^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Articular cartilage is exposed to a gradient of oxygen levels ranging from 5% at the surface to 1% in the deepest layers. While most cartilage research is performed in supra physiological oxygen levels (19-21%), culturing chondrocytes under hypoxic oxygen levels (

Original language	English
Pages (from-to)	9438-9444
Number of pages	7
Journal	Analytical Chemistry
Volume	89
Issue number	17
DOIs	https://doi.org/10.1021/acs.analchem.7b02265
Publication status	Published - 5 Sept 2017

Keywords

ION MASS-SPECTROMETRY
MITOCHONDRIAL CARDIOLIPIN
HYPOXIA
CARTILAGE
DIFFERENTIATION
METABOLISM
TENSION
PATHOPHYSIOLOGY
CHONDROGENESIS
OSTEOARTHRITIS

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10.1021/acs.analchem.7b02265Licence: CC BY-NC-ND

Cite this

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title = "Oxygen-Dependent Lipid Profiles of Three-Dimensional Cultured Human Chondrocytes Revealed by MALDI-MSI",

abstract = "Articular cartilage is exposed to a gradient of oxygen levels ranging from 5% at the surface to 1% in the deepest layers. While most cartilage research is performed in supra physiological oxygen levels (19-21%), culturing chondrocytes under hypoxic oxygen levels (",

keywords = "ION MASS-SPECTROMETRY, MITOCHONDRIAL CARDIOLIPIN, HYPOXIA, CARTILAGE, DIFFERENTIATION, METABOLISM, TENSION, PATHOPHYSIOLOGY, CHONDROGENESIS, OSTEOARTHRITIS",

author = "Brenda Bakker and Eijkel, {Gert B.} and Heeren, {Ron M. A.} and Marcel Karperien and Post, {Janine N.} and Berta Cillero-Pastor",

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AU - Heeren, Ron M. A.

AU - Karperien, Marcel

AU - Post, Janine N.

AU - Cillero-Pastor, Berta

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KW - MITOCHONDRIAL CARDIOLIPIN

KW - HYPOXIA

KW - CARTILAGE

KW - DIFFERENTIATION

KW - METABOLISM

KW - TENSION

KW - PATHOPHYSIOLOGY

KW - CHONDROGENESIS

KW - OSTEOARTHRITIS

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