Oxime Catalysis by Freezing

Stijn M. Agten, Dennis P. L. Suylen, Tilman M. Hackeng*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Chemical reaction rates are generally decreased at lower temperatures. Here, we report that an oxime ligation reaction in water at neutral pH is accelerated by freezing. The freezing method and its rate effect on oxime ligation are systematically studied on a peptide model system, and applied to a larger chemokine protein, containing a single acetyl butyrate group, which is conjugated to an aminooxy-labeled ligand. Our improved ligation protocol now makes it possible to efficiently introduce oxime-bond coupled ligands into proteins under aqueous conditions at low concentrations and neutral pH.
Original languageEnglish
Pages (from-to)42-46
JournalBioconjugate Chemistry
Issue number1
Publication statusPublished - Jan 2016

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