Overexpression of arginase I in enterocytes of transgenic mice elicits a selective arginine deficiency and affects skin, muscle, and lymphoid development

W.J. de Jonge, M.M. Hallemeesch, K.L. Kwikkers, J.M. Ruijter, C. de Gier de-Vries, M.A. van Roon, A.J. Meijer, B. Marescau, N.E.P. Deutz, W.H. Lamers

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Abstract

Overexpression of arginase I in enterocytes of transgenic mice elicits a selective arginine deficiency and affects skin, muscle, and lymphoid development.

de Jonge WJ, Hallemeesch MM, Kwikkers KL, Ruijter JM, de Gier-de Vries C, van Roon MA, Meijer AJ, Marescau B, de Deyn PP, Deutz NE, Lamers WH.

Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, Netherlands.

BACKGROUND: Arginine is required for the detoxification of ammonia and the synthesis of proteins, nitric oxide, agmatine, creatine, and polyamines, and it may promote lymphocyte function. In suckling mammals, arginine is synthesized in the enterocytes of the small intestine, but this capacity is lost after weaning. OBJECTIVE: We investigated the significance of intestinal arginine production for neonatal development in a murine model of chronic arginine deficiency. DESIGN: Two lines of transgenic mice that express different levels of arginase I in their enterocytes were analyzed. RESULTS: Both lines suffer from a selective but quantitatively different reduction in circulating arginine concentration. The degree of arginine deficiency correlated with the degree of retardation of hair and muscle growth and with the development of the lymphoid tissue, in particular Peyer's patches. Expression of arginase in all enterocytes was necessary to elicit this phenotype. Phenotypic abnormalities were reversed by daily injections of arginine but not of creatine. The expression level of the very arginine-rich skin protein trichohyalin was not affected in transgenic mice. Finally, nitric oxide synthase-deficient mice did not show any of the features of arginine deficiency. CONCLUSIONS: Enterocytes are important for maintaining arginine homeostasis in neonatal mice. Graded arginine deficiency causes graded impairment of skin, muscle, and lymphoid development. The effects of arginine deficiency are not mediated by impaired synthesis of creatine or by incomplete charging of arginyl-transfer RNA
Original languageEnglish
Pages (from-to)128-140
Number of pages12
JournalAmerican Journal of Clinical Nutrition
Volume76
Issue number1
DOIs
Publication statusPublished - 1 Jan 2002

Cite this

de Jonge, W. J., Hallemeesch, M. M., Kwikkers, K. L., Ruijter, J. M., de Gier de-Vries, C., van Roon, M. A., Meijer, A. J., Marescau, B., Deutz, N. E. P., & Lamers, W. H. (2002). Overexpression of arginase I in enterocytes of transgenic mice elicits a selective arginine deficiency and affects skin, muscle, and lymphoid development. American Journal of Clinical Nutrition, 76(1), 128-140. https://doi.org/10.1093/ajcn/76.1.128