Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry

Wen-Jia Liu, Lisa Ernst, Benedict Doorschodt, Jan Bednarsch, Felix Becker, Richi Nakatake, Yuki Masano, Ulf Peter Neumann, Sven Arke Lang, Peter Boor, Isabella Lurje, Georg Lurje, Rene Tolba, Zoltan Czigany*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Web of Science)

Abstract

In the present era of organ transplantation with critical organ shortage, various strategies are employed to expand the pool of available allografts for kidney transplantation (KT). Even though, the use of allografts from extended criteria donors (ECD) could partially ease the shortage of organ donors, ECD organs carry a potentially higher risk for inferior outcomes and postoperative complications. Dynamic organ preservation techniques, modulation of ischemia-reperfusion and preservation injury, and allograft therapies are in the spotlight of scientific interest in an effort to improve allograft utilization and patient outcomes in KT.

Preclinical animal experiments are playing an essential role in translational research, especially in the medical device and drug development. The major advantage of the porcine orthotopic auto-transplantation model over ex vivo or small animal studies lies within the surgical-anatomical and physiological similarities to the clinical setting. This allows the investigation of new therapeutic methods and techniques and ensures a facilitated clinical translation of the findings. This protocol provides a comprehensive and problem-oriented description of the porcine orthotopic kidney auto-transplantation model, using a preservation time of 24 hours and telemetry monitoring. The combination of sophisticated surgical techniques with highly standardized and state-of-the-art methods of anesthesia, animal housing, perioperative follow up, and monitoring ensure the reproducibility and success of this model.

Original languageEnglish
Article number61591
Number of pages21
JournalJournal of visualized experiment
Issue number162
DOIs
Publication statusPublished - Aug 2020

Keywords

  • TRANSPLANT RECIPIENTS
  • SEVERITY ASSESSMENT
  • MACHINE PERFUSION
  • SURVIVAL

Cite this