Origin, distribution and 3D-modeling of Gr-EXPB1, an expansin from the potato cyst nematode Globodera rostochiensis

U. Kudla; L. Qin; A. Milac; A. Kielak; C. Maissen; H. Overmars; H.E. Popeijus; E. Roze; A. Petrescu; G. Smant; J. de Bakker; J. Helder

doi:10.1016/j.febslet.2005.03.047

Origin, distribution and 3D-modeling of Gr-EXPB1, an expansin from the potato cyst nematode Globodera rostochiensis

U. Kudla, L. Qin, A. Milac, A. Kielak, C. Maissen, H. Overmars, H.E. Popeijus, E. Roze, A. Petrescu, G. Smant, J. de Bakker, J. Helder^*

^*Corresponding author for this work

NUTRIM School of Nutrition and Translational Research in Metabolism

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Southern analysis showed that Gr-EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis, is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr-EXPB1 domain 1 (D1) has a flat beta-barrel structure with surface-exposed aromatic rings, whereas the 3D structure of Gr-EXPB1-D2 was remarkably similar to plant expansins. Gr-EXPB1 shows highest sequence similarity to two extracellular proteins from saprophytic soil-inhabiting Actinobacteria, and includes a bacterial type II carbohydrate-binding module. These results support the hypothesis that a number of pathogenicity factors of cyst nematodes is of procaryotic origin and were acquired by horizontal gene transfer.

Original language	English
Pages (from-to)	2451-2457
Journal	Febs Letters
Volume	579
Issue number	11
DOIs	https://doi.org/10.1016/j.febslet.2005.03.047
Publication status	Published - 1 Jan 2005

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10.1016/j.febslet.2005.03.047

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@article{0a9312105e8040a7ab4b15c3ad9a4de7,

title = "Origin, distribution and 3D-modeling of Gr-EXPB1, an expansin from the potato cyst nematode Globodera rostochiensis",

abstract = "Southern analysis showed that Gr-EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis, is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr-EXPB1 domain 1 (D1) has a flat beta-barrel structure with surface-exposed aromatic rings, whereas the 3D structure of Gr-EXPB1-D2 was remarkably similar to plant expansins. Gr-EXPB1 shows highest sequence similarity to two extracellular proteins from saprophytic soil-inhabiting Actinobacteria, and includes a bacterial type II carbohydrate-binding module. These results support the hypothesis that a number of pathogenicity factors of cyst nematodes is of procaryotic origin and were acquired by horizontal gene transfer.",

author = "U. Kudla and L. Qin and A. Milac and A. Kielak and C. Maissen and H. Overmars and H.E. Popeijus and E. Roze and A. Petrescu and G. Smant and {de Bakker}, J. and J. Helder",

year = "2005",

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doi = "10.1016/j.febslet.2005.03.047",

language = "English",

volume = "579",

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journal = "Febs Letters",

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TY - JOUR

T1 - Origin, distribution and 3D-modeling of Gr-EXPB1, an expansin from the potato cyst nematode Globodera rostochiensis

AU - Kudla, U.

AU - Qin, L.

AU - Milac, A.

AU - Kielak, A.

AU - Maissen, C.

AU - Overmars, H.

AU - Popeijus, H.E.

AU - Roze, E.

AU - Petrescu, A.

AU - Smant, G.

AU - de Bakker, J.

AU - Helder, J.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Southern analysis showed that Gr-EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis, is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr-EXPB1 domain 1 (D1) has a flat beta-barrel structure with surface-exposed aromatic rings, whereas the 3D structure of Gr-EXPB1-D2 was remarkably similar to plant expansins. Gr-EXPB1 shows highest sequence similarity to two extracellular proteins from saprophytic soil-inhabiting Actinobacteria, and includes a bacterial type II carbohydrate-binding module. These results support the hypothesis that a number of pathogenicity factors of cyst nematodes is of procaryotic origin and were acquired by horizontal gene transfer.

AB - Southern analysis showed that Gr-EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis, is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr-EXPB1 domain 1 (D1) has a flat beta-barrel structure with surface-exposed aromatic rings, whereas the 3D structure of Gr-EXPB1-D2 was remarkably similar to plant expansins. Gr-EXPB1 shows highest sequence similarity to two extracellular proteins from saprophytic soil-inhabiting Actinobacteria, and includes a bacterial type II carbohydrate-binding module. These results support the hypothesis that a number of pathogenicity factors of cyst nematodes is of procaryotic origin and were acquired by horizontal gene transfer.

U2 - 10.1016/j.febslet.2005.03.047

DO - 10.1016/j.febslet.2005.03.047

M3 - Article

C2 - 15848187

SN - 0014-5793

VL - 579

SP - 2451

EP - 2457

JO - Febs Letters

JF - Febs Letters

IS - 11

ER -