Abstract
Introduction: Knowledge of the neuro-anatomical architecture of the inner ear contributes to the improvement and development of cochlear and vestibular implants. The present knowledge ismainly based on two-dimensional images (histology) or derived models that simplify the complexity of this architecture. This study investigated the feasibility of visualizing relevant neuro-anatomical structures of the inner ear in a dynamic three-dimensional reproduction, using a combination of staining, micro-CT imaging and an image processing algorithm. Methods: Four fresh cadaveric temporal bones were postfixed with osmium tetroxide (OsO4) and decalcified with EDTA. Micro-CT was used for scanning at 10 mu m (4 scans) and 5.5 mu m (1 scan) voxel resolution. A new image processing algorithm was developed and the scans were visualized in open source software. Results: OsO4 enhanced the contrast in all scans and the visualization was substantially improved by the image processing algorithm. The three-dimensional renderings provided detailed visualization of the whole inner ear. Details were visible up to the size of individual neurons, nerve crossings and the specific neuro-anatomical structures such as the tunnel of Corti. Conclusion: The combination of OsO4, micro-CT and the proposed image processing algorithm provides an accurate and detailed visualization of the three-dimensional micro-anatomy of the human inner ear.
Original language | English |
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Article number | 41 |
Number of pages | 11 |
Journal | Frontiers in Neuroanatomy |
Volume | 12 |
DOIs | |
Publication status | Published - 22 May 2018 |
Keywords
- contrast enhancement staining
- image processing
- Micro-CT
- temporal bones
- 3D rendering
- inner ear
- cochlear implants
- vestibular implants
- HIGH-RESOLUTION
- TOMOGRAPHY
- STIMULATION
- ANASTOMOSIS
- GEOMETRY
- PLATFORM
- COCHLEA
- SECTION
- TISSUES
- MODELS