Optical Imaging

Frauke Alves, Julia Bode, Peter Cimalla, Ingrid Hilger, Martin Hofmann, Volker Jaedicke, Edmund Koch, Kai Licha, Timo Rademakers, Daniel Razansky, Marc A. M. J. Van Zandvoort

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Abstract

Optical Coherence Tomography (OCT)
We describe the fundamental concept of optical coherence tomography (OCT) and discuss the two main working principles time domain OCT and frequency domain OCT. Then, we review extended functionalities including spectrally and polarization-resolved OCT as well as Doppler-OCT and show concepts for contrast enhancement. Based on these fundamentals, we demonstrate the potential of OCT for small animal imaging on the basis of exemplary studies on retinal imaging and lung imaging.

Optoacoustic Imaging
This chapter deals with the fascinating topic of optoacoustic imaging, a recent powerful addition to the arsenal of in vivo functional and molecular small animal imaging. Due to its hybrid nature, involving optical excitation and ultrasonic detection, optoacoustics overcomes the imaging depth limitations of optical microscopy related to light scattering in living tissues while further benefiting from the compelling advantages of optical contrast. To this end, optoacoustic imaging has been shown capable of delivering multiple types of imaging contrast (structural, functional, kinetic, molecular) within a single imaging modality. It can further deliver images with high spatiotemporal resolution that rivals performance of other well-established whole-body imaging modalities. As such, optoacoustics can play a vital role in biomedical research, from early disease detection and monitoring of dynamic phenomena noninvasively to accelerating drug discovery.

Optical Probes
This chapter is devoted to the properties and application of fluorescence dyes as probes for optical imaging. A variety of agents have been described to date, including nontargeting dyes, vascular agents, targeted conjugates, activatable dyes, and sensing probes. The major classes encompass polymethine dyes and xanthenes dyes, both of which are commercially available in broad variations. Addressing the purpose of optical animal imaging, the most relevant parameters to apply such probes are discussed, thereby supporting the reader in choosing reasonable imaging probes and in preparing bioconjugates for his studies.
Original languageEnglish
Title of host publicationSmall Animal Imaging
Subtitle of host publicationBasics and Practical Guide
EditorsFabian Kiessling, Bernd J. Pichler, Peter Hauff
PublisherSpringer
Chapter16
Pages403-490
Number of pages98
Edition2
ISBN (Electronic)978-3-319-42202-2
ISBN (Print)978-3-319-42200-8
DOIs
Publication statusPublished - 23 May 2017

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