On-tissue chemical derivatization in mass spectrometry imaging

C. Harkin, K.W. Smith, F.L. Cruickshank, C.L. Mackay, B. Flinders, R.M.A. Heeren, T. Moore, S. Brockbank, D.F. Cobice*

*Corresponding author for this work

Research output: Contribution to journal(Systematic) Review article peer-review

17 Citations (Web of Science)

Abstract

Mass spectrometry imaging (MSI) combines molecular and spatial information in a valuable tool for a wide range of applications. Matrix-assisted laser desorption/ionization (MALDI) is at the forefront of MSI ionization due to its wide availability and increasing improvement in spatial resolution and analysis speed. However, ionization suppression, low concentrations, and endogenous and methodological interferences cause visualization problems for certain molecules. Chemical derivatization (CD) has proven a viable solution to these issues when applied in mass spectrometry platforms. Chemical tagging of target analytes with larger, precharged moieties aids ionization efficiency and removes analytes from areas of potential isobaric interferences. Here, we address the application of CD on tissue samples for MSI analysis, termed on-tissue chemical derivatization (OTCD). MALDI MSI will remain the focus platform due to its popularity, however, alternative ionization techniques such as liquid extraction surface analysis and desorption electrospray ionization will also be recognized. OTCD reagent selection, application, and optimization methods will be discussed in detail. MSI with OTCD is a powerful tool to study the spatial distribution of poorly ionizable molecules within tissues. Most importantly, the use of OTCD-MSI facilitates the analysis of previously inaccessible biologically relevant molecules through the adaptation of existing CD methods. Though further experimental optimization steps are necessary, the benefits of this technique are extensive.
Original languageEnglish
Pages (from-to)662-694
Number of pages33
JournalMass Spectrometry Reviews
Volume41
Issue number5
Early online date12 Jan 2021
DOIs
Publication statusPublished - Sep 2022

Keywords

  • ambient conditions
  • atmospheric-pressure
  • bioimaging tof-sims
  • biological tissues
  • chemical derivatization
  • desorption electrospray-ionization
  • maldi-ms
  • mass spectrometry imaging
  • matrix
  • matrix-assisted laser desorption ionization
  • mouse testis
  • silver nanoparticles
  • spatial-resolution
  • MOUSE TESTIS
  • MATRIX
  • MALDI-MS
  • DESORPTION ELECTROSPRAY-IONIZATION
  • SILVER NANOPARTICLES
  • AMBIENT CONDITIONS
  • BIOIMAGING TOF-SIMS
  • SPATIAL-RESOLUTION
  • ATMOSPHERIC-PRESSURE
  • BIOLOGICAL TISSUES

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