On the relationship between molecular mass and anticoagulant activity in a low molecular weight heparin (enoxaparin)

Ana-Victoria Bendetowicz, Elisabeth Pacaud, Suzette Beguin, Andre Uzan, H. Coenraad Hemker

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    Abstract

    A low molecular weight heparin (enoxaparin, mean molecular weight ~ 4,400) was separated by gel chromatography into eight different fractions with a narrow distribution around the following mean molecular weights: 1,800, 2,400, 2,900, 4,200, 6,200, 8,600, 9,800 and 11,000. We compared the influence of enoxaparin on the generation of thrombin in plasma to that of the eight fractions. We determined: a) the % of material with high affinity to antithrombin III (HAM) and the % of HAM above the critical chainlength necessary to allow for thrombin inhibition (ACLM), b) the specific catalytic activity on the decay of endogenous thrombin, and c) the inhibition of over-all thrombin formation in the extrinsic and the intrinsic pathway. From b and c we calculated the inhibition of prothrombin conversion in these pathways. We found that a) there is a gradual decrease of the HAM fraction with decreasing molecular weight; b) the specific catalytic activity for the inactivation of thrombin does not vary significantly between the fractions when expressed in terms of ACLM; c) the potency to inhibit prothrombin conversion does not vary significantly between the fractions when expressed in terms of HAM.
    Original languageEnglish
    Pages (from-to)556-562
    Number of pages7
    JournalThrombosis and Haemostasis
    Volume67
    Issue number5
    DOIs
    Publication statusPublished - 1991

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