Object recognition testing: Rodent species, strains, housing conditions, and estrous cycle

M. van Goethem, K. Rutten, F.J. van der Staay, L.A.W. Jans, S. Akkerman, H.W.M. Steinbusch, A. Blokland, J. van 't Klooster, J. Prickaerts

Research output: Contribution to journalArticleAcademicpeer-review

42 Citations (Scopus)

Abstract

The object recognition task (ORT) allows assessing learning and memory processes in rodents. In this study, two areas in which knowledge about the ORT could be extended were addressed; i.e. generality to species and strains, and intervening variables including housing and estrous cycle. Regarding generality to species and strains, the ORT performance of golden hamsters was assessed. The hamsters showed sufficient exploration times, object recognition performance, and a retention-interval dependent decline similar to rats and mice. Subsequently, we tested three mouse strains which have not been described before in the ORT; i.e. OF1, NMRI, and SJL mice. OF1 and NMRI strains performed equally well, whereas the SJL strain showed low exploration times and no memory retention. Therefore, the SJL strain is unsuited for ORT experiments using a 1 h retention interval and a fixed (3 min) trial duration. Furthermore, the sensitivity to a pharmacological memory deficit model (scopolamine) was tested in three rat strains. Each strain showed a dose dependent relationship, but the least effective dose of scopolamine differed among the three strains, the effect being greater in the order of Wistar, Long-Evans, Hooded Lister rats. Finally, to investigate potential intervening variables in the ORT, the effects of housing conditions and estrous cycle were investigated with rats. Single housing resulted in absolute higher performance than social housing. Furthermore, females in pro-estrus/estrus showed better performance compared to females in metestrus/di-estrus. Taken together, object recognition appears to be a common ability of rodent species, but different strains have different memory capacities and sensitivities to scopolamine, individual housing leads to higher performance, and performance of females is dependent on the estrous cycle phase. Thus, rodent species, strain, housing, and estrous cycle should be taken into consideration in ORT studies. (C) 2012 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)323-334
JournalBehavioural Brain Research
Volume232
Issue number2
DOIs
Publication statusPublished - 1 Jan 2012

Cite this

van Goethem, M. ; Rutten, K. ; van der Staay, F.J. ; Jans, L.A.W. ; Akkerman, S. ; Steinbusch, H.W.M. ; Blokland, A. ; van 't Klooster, J. ; Prickaerts, J. / Object recognition testing: Rodent species, strains, housing conditions, and estrous cycle. In: Behavioural Brain Research. 2012 ; Vol. 232, No. 2. pp. 323-334.
@article{428c788063aa4f21ab8f75b6b2e37363,
title = "Object recognition testing: Rodent species, strains, housing conditions, and estrous cycle",
abstract = "The object recognition task (ORT) allows assessing learning and memory processes in rodents. In this study, two areas in which knowledge about the ORT could be extended were addressed; i.e. generality to species and strains, and intervening variables including housing and estrous cycle. Regarding generality to species and strains, the ORT performance of golden hamsters was assessed. The hamsters showed sufficient exploration times, object recognition performance, and a retention-interval dependent decline similar to rats and mice. Subsequently, we tested three mouse strains which have not been described before in the ORT; i.e. OF1, NMRI, and SJL mice. OF1 and NMRI strains performed equally well, whereas the SJL strain showed low exploration times and no memory retention. Therefore, the SJL strain is unsuited for ORT experiments using a 1 h retention interval and a fixed (3 min) trial duration. Furthermore, the sensitivity to a pharmacological memory deficit model (scopolamine) was tested in three rat strains. Each strain showed a dose dependent relationship, but the least effective dose of scopolamine differed among the three strains, the effect being greater in the order of Wistar, Long-Evans, Hooded Lister rats. Finally, to investigate potential intervening variables in the ORT, the effects of housing conditions and estrous cycle were investigated with rats. Single housing resulted in absolute higher performance than social housing. Furthermore, females in pro-estrus/estrus showed better performance compared to females in metestrus/di-estrus. Taken together, object recognition appears to be a common ability of rodent species, but different strains have different memory capacities and sensitivities to scopolamine, individual housing leads to higher performance, and performance of females is dependent on the estrous cycle phase. Thus, rodent species, strain, housing, and estrous cycle should be taken into consideration in ORT studies. (C) 2012 Elsevier B.V. All rights reserved.",
author = "{van Goethem}, M. and K. Rutten and {van der Staay}, F.J. and L.A.W. Jans and S. Akkerman and H.W.M. Steinbusch and A. Blokland and {van 't Klooster}, J. and J. Prickaerts",
year = "2012",
month = "1",
day = "1",
doi = "10.1016/j.bbr.2012.03.023",
language = "English",
volume = "232",
pages = "323--334",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier Science",
number = "2",

}

Object recognition testing: Rodent species, strains, housing conditions, and estrous cycle. / van Goethem, M.; Rutten, K.; van der Staay, F.J.; Jans, L.A.W.; Akkerman, S.; Steinbusch, H.W.M.; Blokland, A.; van 't Klooster, J.; Prickaerts, J.

In: Behavioural Brain Research, Vol. 232, No. 2, 01.01.2012, p. 323-334.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Object recognition testing: Rodent species, strains, housing conditions, and estrous cycle

AU - van Goethem, M.

AU - Rutten, K.

AU - van der Staay, F.J.

AU - Jans, L.A.W.

AU - Akkerman, S.

AU - Steinbusch, H.W.M.

AU - Blokland, A.

AU - van 't Klooster, J.

AU - Prickaerts, J.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - The object recognition task (ORT) allows assessing learning and memory processes in rodents. In this study, two areas in which knowledge about the ORT could be extended were addressed; i.e. generality to species and strains, and intervening variables including housing and estrous cycle. Regarding generality to species and strains, the ORT performance of golden hamsters was assessed. The hamsters showed sufficient exploration times, object recognition performance, and a retention-interval dependent decline similar to rats and mice. Subsequently, we tested three mouse strains which have not been described before in the ORT; i.e. OF1, NMRI, and SJL mice. OF1 and NMRI strains performed equally well, whereas the SJL strain showed low exploration times and no memory retention. Therefore, the SJL strain is unsuited for ORT experiments using a 1 h retention interval and a fixed (3 min) trial duration. Furthermore, the sensitivity to a pharmacological memory deficit model (scopolamine) was tested in three rat strains. Each strain showed a dose dependent relationship, but the least effective dose of scopolamine differed among the three strains, the effect being greater in the order of Wistar, Long-Evans, Hooded Lister rats. Finally, to investigate potential intervening variables in the ORT, the effects of housing conditions and estrous cycle were investigated with rats. Single housing resulted in absolute higher performance than social housing. Furthermore, females in pro-estrus/estrus showed better performance compared to females in metestrus/di-estrus. Taken together, object recognition appears to be a common ability of rodent species, but different strains have different memory capacities and sensitivities to scopolamine, individual housing leads to higher performance, and performance of females is dependent on the estrous cycle phase. Thus, rodent species, strain, housing, and estrous cycle should be taken into consideration in ORT studies. (C) 2012 Elsevier B.V. All rights reserved.

AB - The object recognition task (ORT) allows assessing learning and memory processes in rodents. In this study, two areas in which knowledge about the ORT could be extended were addressed; i.e. generality to species and strains, and intervening variables including housing and estrous cycle. Regarding generality to species and strains, the ORT performance of golden hamsters was assessed. The hamsters showed sufficient exploration times, object recognition performance, and a retention-interval dependent decline similar to rats and mice. Subsequently, we tested three mouse strains which have not been described before in the ORT; i.e. OF1, NMRI, and SJL mice. OF1 and NMRI strains performed equally well, whereas the SJL strain showed low exploration times and no memory retention. Therefore, the SJL strain is unsuited for ORT experiments using a 1 h retention interval and a fixed (3 min) trial duration. Furthermore, the sensitivity to a pharmacological memory deficit model (scopolamine) was tested in three rat strains. Each strain showed a dose dependent relationship, but the least effective dose of scopolamine differed among the three strains, the effect being greater in the order of Wistar, Long-Evans, Hooded Lister rats. Finally, to investigate potential intervening variables in the ORT, the effects of housing conditions and estrous cycle were investigated with rats. Single housing resulted in absolute higher performance than social housing. Furthermore, females in pro-estrus/estrus showed better performance compared to females in metestrus/di-estrus. Taken together, object recognition appears to be a common ability of rodent species, but different strains have different memory capacities and sensitivities to scopolamine, individual housing leads to higher performance, and performance of females is dependent on the estrous cycle phase. Thus, rodent species, strain, housing, and estrous cycle should be taken into consideration in ORT studies. (C) 2012 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.bbr.2012.03.023

DO - 10.1016/j.bbr.2012.03.023

M3 - Article

VL - 232

SP - 323

EP - 334

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

IS - 2

ER -