Obesity shows preserved plasma proteome in large independent clinical cohorts

Ornella Cominetti, Antonio Nunez Galindo, John Corthesy, Armand Valsesia, Irina Irincheeva, Martin Kussmann, Wim H. M. Saris, Arne Astrup, Ruth McPherson, Mary-Ellen Harper, Robert Dent, Jorg Hager, Loic Dayon*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Holistic human proteome maps are expected to complement comprehensive profile assessment of health and disease phenotypes. However, methodologies to analyze proteomes in human tissue or body fluid samples at relevant scale and performance are still limited in clinical research. Their deployment and demonstration in large enough human populations are even sparser. In the present study, we have characterized and compared the plasma proteomes of two large independent cohorts of obese and overweight individuals using shotgun mass spectrometry (MS)-based proteomics. Herein, we showed, in both populations from different continents of about 500 individuals each, the concordance of plasma protein MS measurements in terms of variability, gender-specificity, and age-relationship. Additionally, we replicated several known and new associations between proteins, clinical and molecular variables, such as insulin and glucose concentrations. In conclusion, our MS-based analyses of plasma samples from independent human cohorts proved the practical feasibility and efficiency of a large and unified discovery/replication approach in proteomics, which was also recently coined "rectangular" design.
Original languageEnglish
Article number16981
Number of pages13
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 16 Nov 2018

Keywords

  • C-REACTIVE PROTEIN
  • BIOMARKER DISCOVERY
  • GENDER-DIFFERENCES
  • MISSING VALUES
  • MASS
  • WORKFLOW
  • SERUM
  • SCALE
  • FIELD

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