Notecarin D Binds Human Factor V and Factor V-a with High Affinity in the Absence of Membranes

Jennifer L. Newell-Caito, Malabika Laha, Anthony C. Tharp, Jonathan I. Creamer, Hong Xu, Ashoka A. Maddur, Guido Tans, Paul E. Bock*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Notecarin D (NotD) is a prothrombin (ProT) activator in the venom of the tiger snake, Notechis scutatus, and a factor Xa (FXa) homolog. NotD binds specifically to the FXa binding site expressed on factor V (FV) upon activation to factor Va (FVa) by thrombin. NotD active site-labeled with 5-fluorescein ([5F]FFR-NotD) binds FV and FVa with remarkably high affinity in the absence of phospholipids (K-D 12 and
Original languageEnglish
Pages (from-to)38286-38297
JournalJournal of Biological Chemistry
Volume286
Issue number44
DOIs
Publication statusPublished - 4 Nov 2011

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