Notecarin D Binds Human Factor V and Factor V-a with High Affinity in the Absence of Membranes

Jennifer L. Newell-Caito; Malabika Laha; Anthony C. Tharp; Jonathan I. Creamer; Hong Xu; Ashoka A. Maddur; Guido Tans; Paul E. Bock

doi:10.1074/jbc.M111.247122

Notecarin D Binds Human Factor V and Factor V-a with High Affinity in the Absence of Membranes

Jennifer L. Newell-Caito, Malabika Laha, Anthony C. Tharp, Jonathan I. Creamer, Hong Xu, Ashoka A. Maddur, Guido Tans, Paul E. Bock^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Web of Science)

Abstract

Notecarin D (NotD) is a prothrombin (ProT) activator in the venom of the tiger snake, Notechis scutatus, and a factor Xa (FXa) homolog. NotD binds specifically to the FXa binding site expressed on factor V (FV) upon activation to factor Va (FVa) by thrombin. NotD active site-labeled with 5-fluorescein ([5F]FFR-NotD) binds FV and FVa with remarkably high affinity in the absence of phospholipids (K-D 12 and

Original language	English
Pages (from-to)	38286-38297
Journal	Journal of Biological Chemistry
Volume	286
Issue number	44
DOIs	https://doi.org/10.1074/jbc.M111.247122
Publication status	Published - 4 Nov 2011

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10.1074/jbc.M111.247122

Cite this

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title = "Notecarin D Binds Human Factor V and Factor V-a with High Affinity in the Absence of Membranes",

abstract = "Notecarin D (NotD) is a prothrombin (ProT) activator in the venom of the tiger snake, Notechis scutatus, and a factor Xa (FXa) homolog. NotD binds specifically to the FXa binding site expressed on factor V (FV) upon activation to factor Va (FVa) by thrombin. NotD active site-labeled with 5-fluorescein ([5F]FFR-NotD) binds FV and FVa with remarkably high affinity in the absence of phospholipids (K-D 12 and ",

author = "Newell-Caito, {Jennifer L.} and Malabika Laha and Tharp, {Anthony C.} and Creamer, {Jonathan I.} and Hong Xu and Maddur, {Ashoka A.} and Guido Tans and Bock, {Paul E.}",

year = "2011",

month = nov,

day = "4",

doi = "10.1074/jbc.M111.247122",

language = "English",

volume = "286",

pages = "38286--38297",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology, Inc.",

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TY - JOUR

T1 - Notecarin D Binds Human Factor V and Factor V-a with High Affinity in the Absence of Membranes

AU - Newell-Caito, Jennifer L.

AU - Laha, Malabika

AU - Tharp, Anthony C.

AU - Creamer, Jonathan I.

AU - Xu, Hong

AU - Maddur, Ashoka A.

AU - Tans, Guido

AU - Bock, Paul E.

PY - 2011/11/4

Y1 - 2011/11/4

N2 - Notecarin D (NotD) is a prothrombin (ProT) activator in the venom of the tiger snake, Notechis scutatus, and a factor Xa (FXa) homolog. NotD binds specifically to the FXa binding site expressed on factor V (FV) upon activation to factor Va (FVa) by thrombin. NotD active site-labeled with 5-fluorescein ([5F]FFR-NotD) binds FV and FVa with remarkably high affinity in the absence of phospholipids (K-D 12 and

AB - Notecarin D (NotD) is a prothrombin (ProT) activator in the venom of the tiger snake, Notechis scutatus, and a factor Xa (FXa) homolog. NotD binds specifically to the FXa binding site expressed on factor V (FV) upon activation to factor Va (FVa) by thrombin. NotD active site-labeled with 5-fluorescein ([5F]FFR-NotD) binds FV and FVa with remarkably high affinity in the absence of phospholipids (K-D 12 and

U2 - 10.1074/jbc.M111.247122

DO - 10.1074/jbc.M111.247122

M3 - Article

C2 - 21911491

SN - 0021-9258

VL - 286

SP - 38286

EP - 38297

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 44

ER -