Nitroimidazole-based inhibitors DTP338 and DTP348 are safe for zebrafish embryos and efficiently inhibit the activity of human CA IX in Xenopus oocytes

Ashok Aspatwar*, Holger M. Becker, Nanda Kumar Parvathaneni, Milka Hammaren, Aleksandra Svorjova, Harlan Barker, Claudiu T. Supuran, Ludwig Dubois, Philippe Lambin, Mataleena Parikka, Seppo Parkkila, Jean-Yves Winum

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Web of Science)

Abstract

Carbonic anhydrase (CA) IX is a hypoxia inducible enzyme that is highly expressed in solid tumours. Therefore, it has been considered as an anticancer target using specific chemical inhibitors. The nitroimidazoles DTP338 and DTP348 have been shown to inhibit CA IX in nanomolar range in vitro and reduce extracellular acidification in hypoxia, and impair tumour growth. We screened these compounds for toxicity using zebrafish embryos and measured their in vivo effects on human CA IX in Xenopus oocytes. In the toxicity screening, the LD50 for both compounds was 3.5mM. Neither compound showed apparent toxicity below 300 mu M concentration. Above this concentration, both compounds altered the movement of zebrafish larvae. The IC50 was 0.14 +/- 0.02 mu M for DTP338 and 19.26 +/- 1.97 mu M for DTP348, suggesting that these compounds efficiently inhibit CA IX in vivo. Our results suggest that these compounds can be developed as drugs for cancer therapy.
Original languageEnglish
Pages (from-to)1064-1073
Number of pages10
JournalJournal of Enzyme Inhibition and Medicinal Chemistry
Volume33
Issue number1
DOIs
Publication statusPublished - 17 Jun 2018

Keywords

  • Nitroimidazoles
  • carbonic anhydrase IX
  • zebrafish
  • Xenopus oocytes
  • in vivo inhibition
  • CARBONIC-ANHYDRASE-IX
  • CANCER-THERAPY
  • PH REGULATION
  • ISOZYME-IX
  • TOXICITY
  • HYPOXIA
  • MODEL
  • ETHOXZOLAMIDE
  • TUBERCULOSIS
  • BICARBONATE

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