New metallothionein mRNAs in Gobio gobio reveal at least three gene duplication events in cyprinid metallothionein evolution

D Knapen*, E Steen Redeker, Inacio, W De Coen, E Verheyen, R Blust

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Web of Science)

Abstract

This paper reports the identification and analysis of the primary structure of three. novel metallothionein cDNA sequences in the gudgeon, Gobio gobio (Cyprinidae), Two different 180 bp coding regions were identified, resulting in two MT isoforms differing in one amino acid. The primary structure of the amino acid sequence was compared to other cyprinid NIT sequences. Furthermore, two differently sized cDNAs were discovered in one of the two transcripts. We present a phylogenetic comparison of our sequences to other, previously published cyprinid NIT gene sequences. Our analysis reveals an unexpected complexity in cyprinid MT evolution, with at least three gene duplication events. Differences and homologies between the evolution of cyprinid NIT genes are compared to other teleost families. Finally, possible implications for metallothionein classification are discussed: (c) 2005 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)347-355
Number of pages9
JournalComparative Biochemistry and Physiology C-toxicology & Pharmacology
Volume140
Issue number3-4
DOIs
Publication statusPublished - 2005
Externally publishedYes

Keywords

  • cDNA
  • cloning
  • cyprinidae
  • gene duplication
  • gene evolution
  • gobio gobio
  • metallothionein
  • phylogeny
  • sequencing
  • CARP CARASSIUS-CUVIERI
  • PIMEPHALES-PROMELAS
  • SEQUENCE ALIGNMENT
  • RAINBOW-TROUT
  • CDNA CLONING
  • COMMON CARP
  • EXPRESSION
  • CADMIUM
  • LOCALIZATION
  • INDUCTION

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