Neutrophils cause oxidative DNA damage in alveolar epithelial cells.

P.A.E.L. Schilderman-Houba, P.J.A. Borm, A.M. Knaapen, F. Seiler, P. Nehls, J. Bruch, R.P.F. Schins

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Department of Health Risk Analysis and Toxicology, Maastricht University, The Netherlands.

Inflammation has been recognized as a contributing factor in the pathogenesis of some cancers. In the lung, inflammation is characterized by an influx of polymorphonuclear leukocytes (PMN) that release a variety of reactive oxygen species (ROS). The aim of the present study was to investigate the direct effect of PMN on oxidative DNA damage in lung target cells. Therefore, rat alveolar epithelial cells (RLE) were coincubated with PMN or hydrogen peroxide. Known to be correlated with the incidence of cancer, 7-hydro-8-oxo-2'deoxyguanosine (8-oxodG) was used as an effect marker for oxidative damage. Viability of the RLE, when coincubated with PMN, decreased to 43%, dependent on the ratio between PMN and RLE. After washing off PMN, 8-oxodG levels were significantly increased in RLE, but the highest levels were observed in the washed off PMN fraction. In addition, to avoid washing off procedures, immunohistochemical analysis was used to measure the 8-oxodG levels specifically in the RLE and similar results were obtained. In addition, inhibitor experiments showed that antioxidants ameliorated oxidative DNA damage. Our data provide evidence that ROS released by PMN as well as H2O2, cause oxidative DNA damage in epithelial cells.
Original languageEnglish
Pages (from-to)234-240
Number of pages7
JournalFree Radical Biology and Medicine
Volume27
Issue number1-2
DOIs
Publication statusPublished - 1 Jan 1999

Cite this