Nephrotoxicity in Mice After Repeated Imaging Using In-111-Labeled Peptides

Marleen Melis*, Erik Vegt, Mark W. Konijnenberg, Monique de Visser, Magda Bijster, Marcel Vermeij, Eric P. Krenning, Otto C. Boerman, Marion de Jong

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Web of Science)


We determined the renal radiation dose of a series of (111)In-labeled peptides using animal SPECT. Because the animals' health deteriorated, renal toxicity was assessed.Wild-type and megalin-deficient mice were imaged repeatedly at 3- to 6-wk intervals to quantify renal retention after injection of 40-50 MBq of (111)In-diethylenetriaminepentaacetic acid-labeled peptides (octreotide, exendin, octreotate, neurotensin, and minigastrin analogs), and the absorbed kidney radiation doses were estimated. Body weight, renal function parameters, and renal histology were determined at 16-20 wk after the first scan and compared with those in naive animals.Because of high renal retention, (111)In-diethylenetriaminepentaacetic acid-exendin-4 scans resulted in a 70-Gy kidney radiation dose in wild-type mice. Megalin-deficient kidneys received 20-40 Gy. The other peptides resulted in much lower renal doses. Kidney function monitoring indicated renal damage in imaged animals.Micro-SPECT enables longitudinal studies in 1 animal. However, long-term nephrotoxic effects may be induced after high renal radiation doses, even with (111)In-labeled radiotracers.
Original languageEnglish
Pages (from-to)973-977
JournalJournal of Nuclear Medicine
Issue number6
Publication statusPublished - 1 Jun 2010


  • nephrotoxicity
  • In-111
  • renal reabsorption
  • radiolabeled peptide
  • dosimetry

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