The recent separation of non-eating disordered obesity into a subtype that is high in negative affect and a subtype that is low in negative affect led to the hypothesis that the two subtypes would show opposite eating responses to typical triggers of overeating. Overweight/obese and normal weight participants, clustered into high and low negative affect subtypes, took part in an experiment using a control condition and two typically disinhibiting manipulations: negative mood induction and tasty food exposure. In accordance with the hypothesis, the negative mood induction and the food exposure elicited overeating in the overweight/obese high negative affect subtype. The overweight/obese low negative affect subtype did not eat more after negative mood induction and food exposure than without a trigger for overeating. Likewise, the normal weight participants did not show differential responses to the three manipulations. The increased vulnerability to overeating in this non-eating disordered overweight/obese subtype that is characterized by increased negative affect shows that individual differences play a crucial role in the way overweight/obese people handle temptations of the current environment. Being characterized by high negative affect makes it more difficult for the overweight/obese to resist temptations. Future studies into non-eating disordered obesity should consider the existence of these two subtypes.