Nanoscopic visualization of microgel-immobilized cytochrome P450 enzymes and their local activity

Microgels provide a controlled microenvironment for enzymes, protecting them from degradation while enhancing stability and activity. Their customizable and biocompatible structure allows for targeted delivery and controlled release, making them ideal for transporting and preserving enzyme function in various applications. For such applications, detailed knowledge of the distribution of enzymes and their activity within the microgels is essential. We present a combination of different localization-based super-resolution fluorescence microscopy measurements to localise single Cytochrome P450 BM3 enzymes and compare their local catalytic activity.The distribution and local catalytic activity of Cytochrome P450 enzymes immobilized in microgels by electrostatic interaction has been determined with a combination of in situ super-resolution fluorescence microscopy experiments.