Myofibrillar and Mitochondrial Protein Synthesis Rates Do Not Differ in Young Men Following the Ingestion of Carbohydrate with Whey, Soy, or Leucine-Enriched Soy Protein after Concurrent Resistance- and Endurance-Type Exercise

Tyler A. Churchward-Venne, Philippe J. M. Pinckaers, Joey S. J. Smeets, Wouter M. Peeters, Antoine H. Zorenc, Henk Schierbeek, Ian Rollo, Lex B. Verdijk, Luc J. C. van Loon*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

18 Citations (Web of Science)

Abstract

Background: Protein ingestion during recovery from resistance-type exercise increases postexercise muscle protein synthesis rates. Whey protein has been reported to have greater anabolic properties than soy protein, an effect which may be attributed to the higher leucine content of whey.

Objective: The objective of this study was to compare postprandial myofibrillar (MyoPS) and mitochondrial (MitoPS) protein synthesis rates after ingestion of carbohydrate with whey, soy, or soy protein enriched with free leucine (to match the leucine content of whey) during recovery from a single bout of concurrent resistance-and endurance-type exercise in young healthy men.

Methods: In a randomized, double-blind, parallel-group design, 36 healthy young recreationally active men (mean +/- SEM age: 23 +/- 0.4 y) received a primed continuous infusion of L-[ring-C-13(6)]-phenylalanine and L-[ring-3,5-H-2(2)]-tyrosine and ingested 45 g carbohydrate with 20 g protein from whey (WHEY), soy (SOY), or leucine-enriched soy (SOY + LEU) after concurrent resistance-and endurance-type exercise. Blood and muscle biopsies were collected over a 360 min postexercise recovery period to assess MyoPS and MitoPS rates, and associated signaling through the mammalian target of rapamycin complex 1 (mTORC1).

Results: Postprandial peak plasma leucine concentrations were significantly higher in WHEY (mean +/- SEM: 322 +/- 10 mu mol/L) and SOY + LEU (328 +/- 14 mu mol/L) compared with SOY (216 +/- 6 mu mol/L) (P <0.05). Despite the apparent differences in plasma leucinemia, MyoPS (WHEY: 0.054 +/- 0.002; SOY: 0.053 +/- 0.004; SOY + LEU: 0.056 +/- 0.004% . h(-1); P = 0.83), and MitoPS (WHEY: 0.061 +/- 0.004; SOY: 0.061 +/- 0.006; SOY + LEU: 0.063 +/- 0.004% . h(-1); P = 0.96) rates over the entire 360 min recovery period did not differ between treatments. Similarly, signaling through mTORC1(Ser2448), p70S6k(Thr389), 4E-BP1(Thr37/46), and rpS6(Ser235/236) was similar between treatments.

Conclusion: Postexercise MyoPS and MitoPS rates do not differ after co-ingestion of carbohydrate with 20 g protein from whey, soy, or leucine-enriched soy protein during 360 min of recovery from concurrent resistance-and endurance-type exercise in young, recreationally active men.

Original languageEnglish
Pages (from-to)210-220
Number of pages11
JournalJournal of Nutrition
Volume149
Issue number2
DOIs
Publication statusPublished - Feb 2019

Keywords

  • muscle protein synthesis
  • young men
  • carbohydrate
  • dietary protein
  • whey
  • soy
  • leucine
  • concurrent exercise
  • HUMAN MUSCLE
  • SKELETAL-MUSCLE
  • AMINO-ACIDS
  • TRANSLATION INITIATION
  • STIMULATION
  • METABOLISM
  • RECOVERY
  • CASEIN
  • KINETICS
  • REST

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