TY - JOUR
T1 - Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents.
AU - Haegens, A.
AU - Vernooy, J.H.
AU - Heeringa, P.
AU - Mossman, B.T.
AU - Wouters, E.F.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - During extensive inflammation, neutrophils undergo secondary necrosis causing myeloperoxidase (MPO) release which may damage resident lung cells. Recent observations suggest that MPO has pro-inflammatory properties, independent of its enzymatic activityHoHHHimportant role in thas. The aims of this study were to characterize MPO internalization by lung epithelial cells and to investigate the effect of MPO on oxidative stress, DNA damage and cytokine production by lung epithelial cells.Human alveolar and bronchial epithelial cells were stimulated with MPO with or without priming the cells with pro-inflammatory stimuli. MPO protein was detected in cell cytoplasm. Expression of heme-oxygenase (HO)-1 and DNA strand breakage were determined. The production of interleukin (IL)-8 and 6 were measured.Analyses of MPO stimulated cells demonstrated MPO presence in the cells. HO-1 expression was increased after MPO stimulation and increased further when cells were primed before MPO stimulation. MPO exposure also induced DNA strand breakage. Interestingly, MPO inhibited IL-8 production in bronchial, but not alveolar epithelium.In conclusion, alveolar and bronchial epithelial cells can internalize MPO. Stimulation with MPO increases HO-1 expression and DNA strand breakage, suggesting cell damaging capacity of MPO. In addition, MPO inhibited IL-8 production by bronchial epithelial cells, indicating a negative feedback loop for neutrophil recruitment.
AB - During extensive inflammation, neutrophils undergo secondary necrosis causing myeloperoxidase (MPO) release which may damage resident lung cells. Recent observations suggest that MPO has pro-inflammatory properties, independent of its enzymatic activityHoHHHimportant role in thas. The aims of this study were to characterize MPO internalization by lung epithelial cells and to investigate the effect of MPO on oxidative stress, DNA damage and cytokine production by lung epithelial cells.Human alveolar and bronchial epithelial cells were stimulated with MPO with or without priming the cells with pro-inflammatory stimuli. MPO protein was detected in cell cytoplasm. Expression of heme-oxygenase (HO)-1 and DNA strand breakage were determined. The production of interleukin (IL)-8 and 6 were measured.Analyses of MPO stimulated cells demonstrated MPO presence in the cells. HO-1 expression was increased after MPO stimulation and increased further when cells were primed before MPO stimulation. MPO exposure also induced DNA strand breakage. Interestingly, MPO inhibited IL-8 production in bronchial, but not alveolar epithelium.In conclusion, alveolar and bronchial epithelial cells can internalize MPO. Stimulation with MPO increases HO-1 expression and DNA strand breakage, suggesting cell damaging capacity of MPO. In addition, MPO inhibited IL-8 production by bronchial epithelial cells, indicating a negative feedback loop for neutrophil recruitment.
U2 - 10.1183/09031936.00029307
DO - 10.1183/09031936.00029307
M3 - Article
C2 - 18057061
SN - 0903-1936
VL - 31
SP - 252
EP - 260
JO - European Respiratory Journal
JF - European Respiratory Journal
IS - 2
ER -