MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study

A. I. Tsonis; P. Zisimopoulou; K. Lazaridis; J. Tzartos; E. Matsigkou; V. Zouvelou; R. Mantegazza; C. Antozzi; F. Andreetta; A. Evoli; F. Deymeer; G. Saruhan-Direskeneli; H. Durmus; T. Brenner; A. Vaknin; S. Berrih-Aknin; A. Behin; T. Sharshar; M. De Baets; M. Losen; P. Martinez-Martinez; K. A. Kleopa; E. Zamba-Papanicolaou; T. Kyriakides; A. Kostera-Pruszczyk; P. Szczudlik; B. Szyluk; D. Lavrnic; I. Basta; S. Peric; C. Tallaksen; A. Maniaol; C. Casasnovas Pons; J. Pitha; M. Jakubikova; F. Hanisch; S. J. Tzartos

doi:10.1016/j.jneuroim.2015.04.015

MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study

A. I. Tsonis, P. Zisimopoulou^*, K. Lazaridis, J. Tzartos, E. Matsigkou, V. Zouvelou, R. Mantegazza, C. Antozzi, F. Andreetta, A. Evoli, F. Deymeer, G. Saruhan-Direskeneli, H. Durmus, T. Brenner, A. Vaknin, S. Berrih-Aknin, A. Behin, T. Sharshar, M. De Baets, M. LosenP. Martinez-Martinez, K. A. Kleopa, E. Zamba-Papanicolaou, T. Kyriakides, A. Kostera-Pruszczyk, P. Szczudlik, B. Szyluk, D. Lavrnic, I. Basta, S. Peric, C. Tallaksen, A. Maniaol, C. Casasnovas Pons, J. Pitha, M. Jakubikova, F. Hanisch, S. J. Tzartos

^*Corresponding author for this work

MHeNs - Neuroscience

Research output: Contribution to journal › Article › Academic › peer-review

42 Citations (Web of Science)

Abstract

Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9% of healthy controls and 5.1% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27% of ocular seronegative patients were MuSK antibody positive. Moreover, 23% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed.

Original language	English
Pages (from-to)	10-17
Journal	Journal of Neuroimmunology
Volume	284
DOIs	https://doi.org/10.1016/j.jneuroim.2015.04.015
Publication status	Published - 15 Jul 2015

Keywords

Myasthenia gravis
MuSK
Autoantibodies
Cell based assay
Diagnosis

Access to Document

10.1016/j.jneuroim.2015.04.015

Cite this

Tsonis, A. I., Zisimopoulou, P., Lazaridis, K., Tzartos, J., Matsigkou, E., Zouvelou, V., Mantegazza, R., Antozzi, C., Andreetta, F., Evoli, A., Deymeer, F., Saruhan-Direskeneli, G., Durmus, H., Brenner, T., Vaknin, A., Berrih-Aknin, S., Behin, A., Sharshar, T., De Baets, M., ... Tzartos, S. J. (2015). MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study. Journal of Neuroimmunology, 284, 10-17. https://doi.org/10.1016/j.jneuroim.2015.04.015

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title = "MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study",

abstract = "Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9% of healthy controls and 5.1% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27% of ocular seronegative patients were MuSK antibody positive. Moreover, 23% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed. ",

keywords = "Myasthenia gravis, MuSK, Autoantibodies, Cell based assay, Diagnosis",

author = "Tsonis, {A. I.} and P. Zisimopoulou and K. Lazaridis and J. Tzartos and E. Matsigkou and V. Zouvelou and R. Mantegazza and C. Antozzi and F. Andreetta and A. Evoli and F. Deymeer and G. Saruhan-Direskeneli and H. Durmus and T. Brenner and A. Vaknin and S. Berrih-Aknin and A. Behin and T. Sharshar and {De Baets}, M. and M. Losen and P. Martinez-Martinez and Kleopa, {K. A.} and E. Zamba-Papanicolaou and T. Kyriakides and A. Kostera-Pruszczyk and P. Szczudlik and B. Szyluk and D. Lavrnic and I. Basta and S. Peric and C. Tallaksen and A. Maniaol and Pons, {C. Casasnovas} and J. Pitha and M. Jakubikova and F. Hanisch and Tzartos, {S. J.}",

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doi = "10.1016/j.jneuroim.2015.04.015",

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Tsonis, AI, Zisimopoulou, P, Lazaridis, K, Tzartos, J, Matsigkou, E, Zouvelou, V, Mantegazza, R, Antozzi, C, Andreetta, F, Evoli, A, Deymeer, F, Saruhan-Direskeneli, G, Durmus, H, Brenner, T, Vaknin, A, Berrih-Aknin, S, Behin, A, Sharshar, T, De Baets, M, Losen, M , Martinez-Martinez, P, Kleopa, KA, Zamba-Papanicolaou, E, Kyriakides, T, Kostera-Pruszczyk, A, Szczudlik, P, Szyluk, B, Lavrnic, D, Basta, I, Peric, S, Tallaksen, C, Maniaol, A, Pons, CC, Pitha, J, Jakubikova, M, Hanisch, F & Tzartos, SJ 2015, 'MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study', Journal of Neuroimmunology, vol. 284, pp. 10-17. https://doi.org/10.1016/j.jneuroim.2015.04.015

TY - JOUR

T1 - MuSK autoantibodies in myasthenia gravis detected by cell based assay - A multinational study

AU - Tsonis, A. I.

AU - Zisimopoulou, P.

AU - Lazaridis, K.

AU - Tzartos, J.

AU - Matsigkou, E.

AU - Zouvelou, V.

AU - Mantegazza, R.

AU - Antozzi, C.

AU - Andreetta, F.

AU - Evoli, A.

AU - Deymeer, F.

AU - Saruhan-Direskeneli, G.

AU - Durmus, H.

AU - Brenner, T.

AU - Vaknin, A.

AU - Berrih-Aknin, S.

AU - Behin, A.

AU - Sharshar, T.

AU - De Baets, M.

AU - Losen, M.

AU - Martinez-Martinez, P.

AU - Kleopa, K. A.

AU - Zamba-Papanicolaou, E.

AU - Kyriakides, T.

AU - Kostera-Pruszczyk, A.

AU - Szczudlik, P.

AU - Szyluk, B.

AU - Lavrnic, D.

AU - Basta, I.

AU - Peric, S.

AU - Tallaksen, C.

AU - Maniaol, A.

AU - Pons, C. Casasnovas

AU - Pitha, J.

AU - Jakubikova, M.

AU - Hanisch, F.

AU - Tzartos, S. J.

PY - 2015/7/15

Y1 - 2015/7/15

N2 - Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9% of healthy controls and 5.1% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27% of ocular seronegative patients were MuSK antibody positive. Moreover, 23% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed.

AB - Seronegative myasthenia gravis (MG) presents a serious gap in MG diagnosis and understanding. We applied a cell based assay (CBA) for the detection of muscle specific kinase (MuSK) antibodies undetectable by radioimmunoassay. We tested 633 triple-seronegative MG patients' sera from 13 countries, detecting 13% as positive. MuSK antibodies were found, at significantly lower frequencies, in 1.9% of healthy controls and 5.1% of other neuroimmune disease patients, including multiple sclerosis and neuromyelitis optica. The clinical data of the newly diagnosed MuSK-MG patients are presented. 27% of ocular seronegative patients were MuSK antibody positive. Moreover, 23% had thymic hyperplasia suggesting that thymic abnormalities are more common than believed.

KW - Myasthenia gravis

KW - MuSK

KW - Autoantibodies

KW - Cell based assay

KW - Diagnosis

U2 - 10.1016/j.jneuroim.2015.04.015

DO - 10.1016/j.jneuroim.2015.04.015

M3 - Article

VL - 284

SP - 10

EP - 17

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

ER -