Abstract
In vivo experiments are accompanied by ethical issues, including sacrificing a large number of animals as well as large costs. A new in vivo 3D screening system was developed to reduce the number of required animals without compromising the results. The present pilot study examined a multiwell array system in combination with three different collagen-based biomaterials (A, B and C) using subcutaneous implantation for 10 days and histological and histomorphometrical evaluations. The tissue reaction towards the device itself was dominated by mononuclear cells. However, three independent biomaterial-specific tissue reactions were observed in three chambers. The results showed a mononuclear cell-based tissue reaction in one chamber (A) and foreign body reaction by multinucleated giant cells in the other two chambers (B and C). Statistical analysis showed a significantly higher number of multinucleated giant cells in cases B and C than in case A (A vs. B; ***P
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Original language | English |
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Article number | 61 |
Number of pages | 6 |
Journal | Journal of Materials Science-Materials in Medicine |
Volume | 30 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2019 |
Keywords
- MULTINUCLEATED GIANT-CELLS
- IMPLANTATION BED
- COLLAGEN MATRIX
- PORCINE DERMIS
- VASCULARIZATION
- MEMBRANES