Multiwell three-dimensional systems enable in vivo screening of immune reactions to biomaterials: a new strategy toward translational biomaterial research

Sarah Al-Maawi, Carlos Mota, Alica Kubesch, C. James Kirkpatrick, Lorenzo Moroni, Shahram Ghanaati*

*Corresponding author for this work

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Abstract

In vivo experiments are accompanied by ethical issues, including sacrificing a large number of animals as well as large costs. A new in vivo 3D screening system was developed to reduce the number of required animals without compromising the results. The present pilot study examined a multiwell array system in combination with three different collagen-based biomaterials (A, B and C) using subcutaneous implantation for 10 days and histological and histomorphometrical evaluations. The tissue reaction towards the device itself was dominated by mononuclear cells. However, three independent biomaterial-specific tissue reactions were observed in three chambers. The results showed a mononuclear cell-based tissue reaction in one chamber (A) and foreign body reaction by multinucleated giant cells in the other two chambers (B and C). Statistical analysis showed a significantly higher number of multinucleated giant cells in cases B and C than in case A (A vs. B; ***P

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Original languageEnglish
Article number61
Number of pages6
JournalJournal of Materials Science-Materials in Medicine
Volume30
Issue number6
DOIs
Publication statusPublished - Jun 2019

Keywords

  • MULTINUCLEATED GIANT-CELLS
  • IMPLANTATION BED
  • COLLAGEN MATRIX
  • PORCINE DERMIS
  • VASCULARIZATION
  • MEMBRANES

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