Multiplex autoantibody detection for autoimmune liver diseases and autoimmune gastritis

Joris Vanderlocht, Mart van der Cruys, Frans Stals, Liesbeth Bakker-Jonges, Jan Damoiseaux*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Autoantibody detection for autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC) and autoimmune gastritis (AIG) is traditionally performed by IIF on a combination of tissues. Multiplex line/dot blots (LIA/DIA) offer multiple advantages, i.e. automation, objective reading, no interfering reactivities, no coincidental findings. In the current study we evaluated automated DIA (D-Tek) for detecting autoantibodies related to autoimmune diseases of the gastrointestinal tract. We tested samples of the Dutch EQC program and compared the results with the consensus of the participating labs. For the autoimmune liver diseases and AIG, respectively, 64 and 36 samples were tested.

For anti-mitochondrial and anti-smooth muscle antibodies a concordance rate of 97% and 88% was observed, respectively. The concordance rate for anti-parietal cell antibodies was 92% when samples without EQC consensus (n=15) were excluded. For antibodies against intrinsic factor a concordance of 96% was observed. For all these antibodies discrepancies were identified that relate to the different test characteristics and the preponderance of IIF utilizing labs in the EQC program.

In conclusion, we observed good agreement of the tested DIA blots with the consensus results of the Dutch EQC program. Taken together with the logistic advantages these blots are a good alternative for autoantibody detection in the respective diseases. A large prospective multicenter study is warranted to position these novel tests further in the whole spectrum of assays for the detection of these antibodies in a routine autoimmune laboratory. (C) 2017 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalJournal of Immunological Methods
Volume448
DOIs
Publication statusPublished - Sept 2017

Keywords

  • Autoantibodies
  • Pernicious anemia
  • Gastritis
  • Hepatitis
  • Primary biliary cirrhosis
  • PRIMARY BILIARY-CIRRHOSIS
  • DIAGNOSIS
  • HEPATITIS
  • IMMUNOFLUORESCENCE
  • CLASSIFICATION
  • ANTIBODIES

Fingerprint

Dive into the research topics of 'Multiplex autoantibody detection for autoimmune liver diseases and autoimmune gastritis'. Together they form a unique fingerprint.

Cite this