Multiple regions of Kaposi's sarcoma-associated herpesvirus ORF59 RNA are required for its expression mediated by viral ORF57 and cellular RBM15

Maria Julia Massimelli, Vladimir Majerciak, Jeong-Gu Kang, David J Liewehr, Seth M Steinberg, Zhi-Ming Zheng*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


KSHV ORF57 (MTA) promotes RNA stability of ORF59, a viral DNA polymerase processivity factor. Here, we show that the integrity of both ORF59 RNA ends is necessary for ORF57-mediated ORF59 expression and deletion of both 5' and 3' regions, or one end region with a central region, of ORF59 RNA prevents ORF57-mediated translation of ORF59. The ORF59 sequence between nt 96633 and 96559 resembles other known MTA-responsive elements (MREs). ORF57 specifically binds to a stem-loop region from nt 96596-96572 of the MRE, which also binds cellular RBM15. Internal deletion of the MRE from ORF59 led to poor export, but accumulation of nuclear ORF59 RNA in the presence of ORF57 or RBM15. Despite of being translatable in the presence of ORF57, this deletion mutant exhibits translational defect in the presence of RBM15. Together, our results provide novel insight into the roles of ORF57 and RBM15 in ORF59 RNA accumulation and protein translation.

Original languageEnglish
Pages (from-to)496-510
Number of pages15
Issue number2
Publication statusPublished - 3 Feb 2015
Externally publishedYes


  • Base Sequence
  • Cell Line
  • Cells, Cultured
  • Epistasis, Genetic
  • Gene Expression Regulation, Viral
  • Herpesviridae Infections/metabolism
  • Herpesvirus 8, Human/genetics
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Protein Biosynthesis
  • RNA Transport
  • RNA, Viral/chemistry
  • RNA-Binding Proteins/metabolism
  • Response Elements
  • Viral Proteins/chemistry
  • Viral Regulatory and Accessory Proteins/chemistry

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