MS Imaging-Guided Microproteomics for Spatial Omics on a Single Instrument

Frederic Dewez, Janina Oejten, Corinna Henkel, Romano Hebeler, Heiko Neuweger, Edwin De Pauw, Ron M. A. Heeren*, Benjamin Balluff

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Mass spectrometry imaging (MSI) allows investigating the spatial distribution of chemical compounds directly in biological tissues. As the analytical depth of MSI is limited, MSI needs to be coupled to more sensitive local extraction-based omics approaches to achieve a comprehensive molecular characterization. For this, it is important to retain the spatial information provided by MSI for follow-up omics studies. It has been shown that regiospecific MSI data can be used to guide a laser microdissection system for ultra-sensitive liquid chromatography-mass spectrometry (LC-MS) analyses. So far, this combination has required separate and specialized mass spectrometry (MS) instrumentation. Recent advances in dual-source instrumentation, harboring both matrix assisted laser/desorption ionization (MALDI) and electrospray ionization (ESI) sources, promise state-of-the-art MSI and liquid-based proteomic capabilities on the same MS instrument. This study demonstrates that such an instrument can offer both fast lipid-based MSI at high mass and high lateral resolution and sensitive LC-MS on local protein extracts from the exact same tissue section.

Original languageEnglish
Article number1900369
Number of pages5
Issue number23
Early online date19 Aug 2020
Publication statusPublished - Dec 2020


  • laser microdissection
  • mass spectrometry imaging
  • microproteomics
  • spatial omics

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