Monitoring the Remodeling of Biohybrid Tissue-Engineered Vascular Grafts by Multimodal Molecular Imaging

Elena Rama, Saurav Ranjan Mohapatra, Christoph Melcher, Teresa Nolte, Seyed Mohammadali Dadfar, Ramona Brueck, Vertika Pathak, Anne Rix, Thomas Gries, Volkmar Schulz, Twan Lammers, Christian Apel, Stefan Jockenhoevel, Fabian Kiessling

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Web of Science)

Abstract

Tissue-engineered vascular grafts (TEVGs) with the ability to grow and remodel open new perspectives for cardiovascular surgery. Equipping TEVGs with synthetic polymers and biological components provides a good compromise between high structural stability and biological adaptability. However, imaging approaches to control grafts' structural integrity, physiological function, and remodeling during the entire transition between late in vitro maturation and early in vivo engraftment are mandatory for clinical implementation. Thus, a comprehensive molecular imaging concept using magnetic resonance imaging (MRI) and ultrasound (US) to monitor textile scaffold resorption, extracellular matrix (ECM) remodeling, and endothelial integrity in TEVGs is presented here. Superparamagnetic iron-oxide nanoparticles (SPION) incorporated in biodegradable poly(lactic-co-glycolic acid) (PLGA) fibers of the TEVGs allow to quantitatively monitor scaffold resorption via MRI both in vitro and in vivo. Additionally, ECM formation can be depicted by molecular MRI using elastin- and collagen-targeted probes. Finally, molecular US of α v β 3 integrins confirms the absence of endothelial dysfunction; the latter is provocable by TNF-α. In conclusion, the successful employment of noninvasive molecular imaging to longitudinally evaluate TEVGs remodeling is demonstrated. This approach may foster its translation from in vitro quality control assessment to in vivo applications to ensure proper prostheses engraftment.

Original languageEnglish
Article number2105783
Pages (from-to)e2105783
Number of pages15
JournalAdvanced Science
Volume9
Issue number10
DOIs
Publication statusPublished - Apr 2022

Keywords

  • Blood Vessel Prosthesis
  • Collagen
  • Extracellular Matrix
  • Molecular Imaging
  • Tissue Engineering/methods
  • IRON-OXIDE NANOPARTICLES
  • GLOBAL BURDEN
  • superpara-magnetic iron-oxide nanoparticles
  • RISK-FACTORS
  • CONTRAST AGENTS
  • alpha(v)beta(3) integrins
  • molecular imaging
  • COLLAGEN SCAFFOLDS
  • tissue-engineering
  • ENDOTHELIAL-CELLS
  • P-SELECTIN
  • MUSCLE CELL-MIGRATION
  • poly(lactic-co-glycolic acid)
  • CARDIOVASCULAR-DISEASES
  • EXPRESSION

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