Monitoring the Initiation and Kinetics of Human Dendritic Cell-Induced Polarization of Autologous Naive CD4(+) T Cells

Tammy Oth, Melanie C. A. Schnijderberg, Birgit L. M. G. Senden-Gijsbers, Wilfred T. V. Germeraad, Gerard M. J. Bos, Joris Vanderlocht*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Web of Science)

Abstract

A crucial step in generating de novo immune responses is the polarization of naive cognate CD4(+) T cells by pathogen-triggered dendritic cells (DC). In the human setting, standardized DC-dependent systems are lacking to study molecular events during the initiation of a naive CD4(+) T cell response. We developed a TCR-restricted assay to compare different pathogen-triggered human DC for their capacities to instruct functional differentiation of autologous, naive CD4(+) T cells. We demonstrated that this methodology can be applied to compare differently matured DC in terms of kinetics, direction, and magnitude of the naive CD4(+) T cell response. Furthermore, we showed the applicability of this assay to study the T cell polarizing capacity of low-frequency blood-derived DC populations directly isolated ex vivo. This methodology for addressing APC-dependent instruction of naive CD4(+) T cells in a human autologous setting will provide researchers with a valuable tool to gain more insight into molecular mechanisms occurring in the early phase of T cell polarization. In addition, it may also allow the study of pharmacological agents on DC-dependent T cell polarization in the human system.
Original languageEnglish
JournalPLOS ONE
Volume9
Issue number8
DOIs
Publication statusPublished - 21 Aug 2014

Cite this