Monitoring platelet dependent thrombin generation in mice

Yesim Dargaud; Henri M. H. Spronk; Peter Leenders; H. Coenraad Hemker; Hugo Ten Cate

doi:10.1016/j.thromres.2010.08.007

Monitoring platelet dependent thrombin generation in mice

Yesim Dargaud^*, Henri M. H. Spronk, Peter Leenders, H. Coenraad Hemker, Hugo Ten Cate

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Calibrated automated thrombin generation assay was adapted to measure thrombin generation in platelet rich plasma from mice. Vena cava phlebotomy appeared the best technique for blood sampling. The concentration-effect curves of tissue factor and platelet count have been determined. Corn trypsin inhibitor 2 mu M inhibits contact activation effectively. Recombinant human thrombomodulin does not inhibit thrombin generation in mouse plasma but activated protein C (20 nM) does. Thrombin generation was dose dependently diminished by low molecular weight heparin and increased by high concentrations of exogenous factor VIII i.e. the assay can detect both hypo-and hypercoagulability.

Original language	English
Pages (from-to)	436-441
Journal	Thrombosis Research
Volume	126
Issue number	5
DOIs	https://doi.org/10.1016/j.thromres.2010.08.007
Publication status	Published - Nov 2010

Keywords

Thrombin generation
Mouse
Platelet
Corn trypsin inhibitor
Activated protein C resistance

Access to Document

10.1016/j.thromres.2010.08.007

Cite this

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title = "Monitoring platelet dependent thrombin generation in mice",

abstract = "Calibrated automated thrombin generation assay was adapted to measure thrombin generation in platelet rich plasma from mice. Vena cava phlebotomy appeared the best technique for blood sampling. The concentration-effect curves of tissue factor and platelet count have been determined. Corn trypsin inhibitor 2 mu M inhibits contact activation effectively. Recombinant human thrombomodulin does not inhibit thrombin generation in mouse plasma but activated protein C (20 nM) does. Thrombin generation was dose dependently diminished by low molecular weight heparin and increased by high concentrations of exogenous factor VIII i.e. the assay can detect both hypo-and hypercoagulability. ",

keywords = "Thrombin generation, Mouse, Platelet, Corn trypsin inhibitor, Activated protein C resistance",

author = "Yesim Dargaud and Spronk, {Henri M. H.} and Peter Leenders and Hemker, {H. Coenraad} and {Ten Cate}, Hugo",

year = "2010",

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language = "English",

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journal = "Thrombosis Research",

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T1 - Monitoring platelet dependent thrombin generation in mice

AU - Dargaud, Yesim

AU - Spronk, Henri M. H.

AU - Leenders, Peter

AU - Hemker, H. Coenraad

AU - Ten Cate, Hugo

PY - 2010/11

Y1 - 2010/11

N2 - Calibrated automated thrombin generation assay was adapted to measure thrombin generation in platelet rich plasma from mice. Vena cava phlebotomy appeared the best technique for blood sampling. The concentration-effect curves of tissue factor and platelet count have been determined. Corn trypsin inhibitor 2 mu M inhibits contact activation effectively. Recombinant human thrombomodulin does not inhibit thrombin generation in mouse plasma but activated protein C (20 nM) does. Thrombin generation was dose dependently diminished by low molecular weight heparin and increased by high concentrations of exogenous factor VIII i.e. the assay can detect both hypo-and hypercoagulability.

AB - Calibrated automated thrombin generation assay was adapted to measure thrombin generation in platelet rich plasma from mice. Vena cava phlebotomy appeared the best technique for blood sampling. The concentration-effect curves of tissue factor and platelet count have been determined. Corn trypsin inhibitor 2 mu M inhibits contact activation effectively. Recombinant human thrombomodulin does not inhibit thrombin generation in mouse plasma but activated protein C (20 nM) does. Thrombin generation was dose dependently diminished by low molecular weight heparin and increased by high concentrations of exogenous factor VIII i.e. the assay can detect both hypo-and hypercoagulability.

KW - Thrombin generation

KW - Mouse

KW - Platelet

KW - Corn trypsin inhibitor

KW - Activated protein C resistance

U2 - 10.1016/j.thromres.2010.08.007

DO - 10.1016/j.thromres.2010.08.007

M3 - Article

C2 - 20843543

SN - 0049-3848

VL - 126

SP - 436

EP - 441

JO - Thrombosis Research

JF - Thrombosis Research

IS - 5

ER -