Monitoring platelet dependent thrombin generation in mice

Yesim Dargaud*, Henri M. H. Spronk, Peter Leenders, H. Coenraad Hemker, Hugo Ten Cate

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Calibrated automated thrombin generation assay was adapted to measure thrombin generation in platelet rich plasma from mice. Vena cava phlebotomy appeared the best technique for blood sampling. The concentration-effect curves of tissue factor and platelet count have been determined. Corn trypsin inhibitor 2 mu M inhibits contact activation effectively. Recombinant human thrombomodulin does not inhibit thrombin generation in mouse plasma but activated protein C (20 nM) does. Thrombin generation was dose dependently diminished by low molecular weight heparin and increased by high concentrations of exogenous factor VIII i.e. the assay can detect both hypo-and hypercoagulability.
Original languageEnglish
Pages (from-to)436-441
JournalThrombosis Research
Issue number5
Publication statusPublished - Nov 2010


  • Thrombin generation
  • Mouse
  • Platelet
  • Corn trypsin inhibitor
  • Activated protein C resistance


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