Monitoring platelet dependent thrombin generation in mice

Yesim Dargaud*, Henri M. H. Spronk, Peter Leenders, H. Coenraad Hemker, Hugo Ten Cate

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Web of Science)

Abstract

Calibrated automated thrombin generation assay was adapted to measure thrombin generation in platelet rich plasma from mice. Vena cava phlebotomy appeared the best technique for blood sampling. The concentration-effect curves of tissue factor and platelet count have been determined. Corn trypsin inhibitor 2 mu M inhibits contact activation effectively. Recombinant human thrombomodulin does not inhibit thrombin generation in mouse plasma but activated protein C (20 nM) does. Thrombin generation was dose dependently diminished by low molecular weight heparin and increased by high concentrations of exogenous factor VIII i.e. the assay can detect both hypo-and hypercoagulability.
Original languageEnglish
Pages (from-to)436-441
JournalThrombosis Research
Volume126
Issue number5
DOIs
Publication statusPublished - Nov 2010

Keywords

  • Thrombin generation
  • Mouse
  • Platelet
  • Corn trypsin inhibitor
  • Activated protein C resistance

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