Monitoring Cell Spheroid Formation And Culture In Porous Membrane Microwells By Electrical Impedance

Alexander P.M. Guttenplan; Maria Gabriella Fois; Thijs Vandenryt; Seppe Bormans; Zeinab Tahmasebi Birgani; Stefan Giselbrecht; Roman K. Truckenmüller; Ronald Thoelen; Pamela Habibovic

Monitoring Cell Spheroid Formation And Culture In Porous Membrane Microwells By Electrical Impedance

Alexander P.M. Guttenplan^*, Maria Gabriella Fois, Thijs Vandenryt, Seppe Bormans, Zeinab Tahmasebi Birgani, Stefan Giselbrecht, Roman K. Truckenmüller, Ronald Thoelen, Pamela Habibovic^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

Abstract

We have constructed a reusable flow cell in which the aggregation of cells in low-attachment microwells to form spheroids can be monitored in real-time using electrical impedance. Culture in dynamic flow conditions allows media to be refreshed without disturbing the cells. The use of transparent ITO electrodes for sensing, and the methods used for fabrication of porous membrane microwells, allow the spheroids to be observed using a microscope. Electrodes for addressable measurement of individual microwells have also been developed.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1701-1702
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - 1 Jan 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Online, Palm Springs, United States Duration: 10 Oct 2021 → 14 Oct 2021 Conference number: 25

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated title	MicroTAS 2021
Country/Territory	United States
City	Palm Springs
Period	10/10/21 → 14/10/21

Keywords

Aggregates
Impedance-Based Sensors
Microthermoforming
Spheroids

Cite this

Guttenplan, A. P. M., Fois, M. G., Vandenryt, T., Bormans, S., Birgani, Z. T., Giselbrecht, S., Truckenmüller, R. K., Thoelen, R., & Habibovic, P. (2021). Monitoring Cell Spheroid Formation And Culture In Porous Membrane Microwells By Electrical Impedance. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1701-1702). Chemical and Biological Microsystems Society.

@inproceedings{120144e23d7243cfaca14f70b8ec01e4,

title = "Monitoring Cell Spheroid Formation And Culture In Porous Membrane Microwells By Electrical Impedance",

abstract = "We have constructed a reusable flow cell in which the aggregation of cells in low-attachment microwells to form spheroids can be monitored in real-time using electrical impedance. Culture in dynamic flow conditions allows media to be refreshed without disturbing the cells. The use of transparent ITO electrodes for sensing, and the methods used for fabrication of porous membrane microwells, allow the spheroids to be observed using a microscope. Electrodes for addressable measurement of individual microwells have also been developed.",

keywords = "Aggregates, Impedance-Based Sensors, Microthermoforming, Spheroids",

author = "Guttenplan, {Alexander P.M.} and Fois, {Maria Gabriella} and Thijs Vandenryt and Seppe Bormans and Birgani, {Zeinab Tahmasebi} and Stefan Giselbrecht and Truckenm{\"u}ller, {Roman K.} and Ronald Thoelen and Pamela Habibovic",

note = "Funding Information: This work was financially supported by the European Union Interreg Vlaanderen-Nederland project “BIOMAT on microfluidic chip” (grant no. 0433). This research has been in part made possible by the Dutch Province of Limburg (program “Limburg INvesteert in haar Kenniseconomie/LINK”; grant nos. SAS-2014-00837 and SAS-2018-02477). SG, RKT and PH gratefully acknowledge the Gravitation Program of the Netherlands Organisation for Scientific Research (NWO) (project {\textquoteleft}Materials-Driven Regeneration{\textquoteright}; grant no. 024.003.013). PH gratefully acknowledges the NWO Vidi grant “Bone Microfactory” (grant no. 15604). ZTB gratefully acknowledges the NWO Incentive Grant for Women in STEM (Project“Biotetris”, 18748). †-these authors contributed equally to this work Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

month = jan,

day = "1",

language = "English",

pages = "1701--1702",

booktitle = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

address = "United States",

}

Guttenplan, APM, Fois, MG, Vandenryt, T, Bormans, S, Birgani, ZT , Giselbrecht, S , Truckenmüller, RK, Thoelen, R & Habibovic, P 2021, Monitoring Cell Spheroid Formation And Culture In Porous Membrane Microwells By Electrical Impedance. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1701-1702, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Palm Springs, United States, 10/10/21.

Monitoring Cell Spheroid Formation And Culture In Porous Membrane Microwells By Electrical Impedance. / Guttenplan, Alexander P.M.; Fois, Maria Gabriella; Vandenryt, Thijs et al.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 1701-1702.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

TY - GEN

T1 - Monitoring Cell Spheroid Formation And Culture In Porous Membrane Microwells By Electrical Impedance

AU - Guttenplan, Alexander P.M.

AU - Fois, Maria Gabriella

AU - Vandenryt, Thijs

AU - Bormans, Seppe

AU - Birgani, Zeinab Tahmasebi

AU - Giselbrecht, Stefan

AU - Truckenmüller, Roman K.

AU - Thoelen, Ronald

AU - Habibovic, Pamela

N1 - Conference code: 25

PY - 2021/1/1

Y1 - 2021/1/1

N2 - We have constructed a reusable flow cell in which the aggregation of cells in low-attachment microwells to form spheroids can be monitored in real-time using electrical impedance. Culture in dynamic flow conditions allows media to be refreshed without disturbing the cells. The use of transparent ITO electrodes for sensing, and the methods used for fabrication of porous membrane microwells, allow the spheroids to be observed using a microscope. Electrodes for addressable measurement of individual microwells have also been developed.

AB - We have constructed a reusable flow cell in which the aggregation of cells in low-attachment microwells to form spheroids can be monitored in real-time using electrical impedance. Culture in dynamic flow conditions allows media to be refreshed without disturbing the cells. The use of transparent ITO electrodes for sensing, and the methods used for fabrication of porous membrane microwells, allow the spheroids to be observed using a microscope. Electrodes for addressable measurement of individual microwells have also been developed.

KW - Aggregates

KW - Impedance-Based Sensors

KW - Microthermoforming

KW - Spheroids

UR - http://www.scopus.com/inward/record.url?scp=85136935339&partnerID=8YFLogxK

M3 - Conference article in proceeding

SP - 1701

EP - 1702

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 10 October 2021 through 14 October 2021

ER -

Monitoring Cell Spheroid Formation And Culture In Porous Membrane Microwells By Electrical Impedance

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Keywords

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