Molecular imaging of alkaloids in khat (Catha edulis) leaves with MeV-SIMS

Bostjan Jencic; Luka Jeromel; Nina Ogrinc Potocnik; Katarina Vogel-Mikus; Primoz Vavpetic; Zdravko Rupnik; Klemen Bucar; Matjaz Vencelj; Mitja Kelemen; Jiro Matsuo; Masakazu Kusakari; Zdravko Siketic; Muhammad A. Al-Jalali; Abdallah Shaltout; Primoz Pelicon

doi:10.1016/j.nimb.2017.01.063

Molecular imaging of alkaloids in khat (Catha edulis) leaves with MeV-SIMS

Bostjan Jencic^*, Luka Jeromel, Nina Ogrinc Potocnik, Katarina Vogel-Mikus, Primoz Vavpetic, Zdravko Rupnik, Klemen Bucar, Matjaz Vencelj, Mitja Kelemen, Jiro Matsuo, Masakazu Kusakari, Zdravko Siketic, Muhammad A. Al-Jalali, Abdallah Shaltout, Primoz Pelicon

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Imaging Mass Spectroscopy (IMS) is a unique research tool providing localization and identification of a wide range of biomolecules as essential data to understand biochemical processes in living organisms. Secondary Ion Mass Spectrometry with high-energy heavy ions (MeV-SIMS) is emerging as a promising IMS technique for chemical imaging of biological tissue.

We measured the molecular mass spatial distributions in leaves of khat (Catha edulis). Khat is a natural drug plant, native to eastern Africa and the Arabian Peninsula. In these countries, fresh leaves are being chewed by significant part of population. It was reported that 80% of the adult population in Yemen chew the khat leaves. The main stimulating effects of khat are induced by a monoamine alkaloid called cathinone. During leaf ageing, cathinone is further metabolised to cathine and norephedrine. Earlier studies identified the alkaloids in khat, however little is known on their spatial distribution, reflecting the biosynthesis and accumulation in the tissue.

Chemical mapping of alkaloids on cross-sections of khat leaves by MeV-SIMS was done at JSI by a pulsed 5.8 MeV Cl-35(6+) beam, focused to a diameter of 15 mu m, using a linear time-of-flight (TOF) spectrometer with a mass resolution of 500. In addition, measurements of MeV-SIMS mass spectra were performed at Kyoto University by a continuous broad beam of 6 MeV Cu-63(4+) ions at an orthogonal TOF spectrometer with a high mass resolution of 11,000. Sections of leaves were analysed and mass spectra obtained at both MeV-SIMS setups were compared. Tissue-level distributions of detected alkaloids are presented and discussed. (C) 2017 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	140-145
Number of pages	6
Journal	Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms
Volume	404
DOIs	https://doi.org/10.1016/j.nimb.2017.01.063
Publication status	Published - 1 Aug 2017
Event	15th International Conference on Nuclear Microprobe Technology and Applications (ICNMTA) / 6th International Workshop on Proton Beam Writing - Lanzhou, China Duration: 31 Jul 2016 → 5 Aug 2016

Keywords

Imaging Mass Spectroscopy
MeV-SIMS
Khat
Nuclear microprobe
MICRO-PIXE

Access to Document

10.1016/j.nimb.2017.01.063

Cite this

Jencic, B., Jeromel, L., Potocnik, N. O., Vogel-Mikus, K., Vavpetic, P., Rupnik, Z., Bucar, K., Vencelj, M., Kelemen, M., Matsuo, J., Kusakari, M., Siketic, Z., Al-Jalali, M. A., Shaltout, A., & Pelicon, P. (2017). Molecular imaging of alkaloids in khat (Catha edulis) leaves with MeV-SIMS. Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms, 404, 140-145. https://doi.org/10.1016/j.nimb.2017.01.063

@article{604b701ab7074bdc90e846a0c680d3f9,

title = "Molecular imaging of alkaloids in khat (Catha edulis) leaves with MeV-SIMS",

abstract = "Imaging Mass Spectroscopy (IMS) is a unique research tool providing localization and identification of a wide range of biomolecules as essential data to understand biochemical processes in living organisms. Secondary Ion Mass Spectrometry with high-energy heavy ions (MeV-SIMS) is emerging as a promising IMS technique for chemical imaging of biological tissue.We measured the molecular mass spatial distributions in leaves of khat (Catha edulis). Khat is a natural drug plant, native to eastern Africa and the Arabian Peninsula. In these countries, fresh leaves are being chewed by significant part of population. It was reported that 80% of the adult population in Yemen chew the khat leaves. The main stimulating effects of khat are induced by a monoamine alkaloid called cathinone. During leaf ageing, cathinone is further metabolised to cathine and norephedrine. Earlier studies identified the alkaloids in khat, however little is known on their spatial distribution, reflecting the biosynthesis and accumulation in the tissue.Chemical mapping of alkaloids on cross-sections of khat leaves by MeV-SIMS was done at JSI by a pulsed 5.8 MeV Cl-35(6+) beam, focused to a diameter of 15 mu m, using a linear time-of-flight (TOF) spectrometer with a mass resolution of 500. In addition, measurements of MeV-SIMS mass spectra were performed at Kyoto University by a continuous broad beam of 6 MeV Cu-63(4+) ions at an orthogonal TOF spectrometer with a high mass resolution of 11,000. Sections of leaves were analysed and mass spectra obtained at both MeV-SIMS setups were compared. Tissue-level distributions of detected alkaloids are presented and discussed. (C) 2017 Elsevier B.V. All rights reserved.",

keywords = "Imaging Mass Spectroscopy, MeV-SIMS, Khat, Nuclear microprobe, MICRO-PIXE",

author = "Bostjan Jencic and Luka Jeromel and Potocnik, {Nina Ogrinc} and Katarina Vogel-Mikus and Primoz Vavpetic and Zdravko Rupnik and Klemen Bucar and Matjaz Vencelj and Mitja Kelemen and Jiro Matsuo and Masakazu Kusakari and Zdravko Siketic and Al-Jalali, {Muhammad A.} and Abdallah Shaltout and Primoz Pelicon",

year = "2017",

month = aug,

day = "1",

doi = "10.1016/j.nimb.2017.01.063",

language = "English",

volume = "404",

pages = "140--145",

journal = "Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms",

issn = "0168-583X",

publisher = "Elsevier Science",

note = "15th International Conference on Nuclear Microprobe Technology and Applications (ICNMTA) / 6th International Workshop on Proton Beam Writing ; Conference date: 31-07-2016 Through 05-08-2016",

}

Jencic, B, Jeromel, L, Potocnik, NO, Vogel-Mikus, K, Vavpetic, P, Rupnik, Z, Bucar, K, Vencelj, M, Kelemen, M, Matsuo, J, Kusakari, M, Siketic, Z, Al-Jalali, MA, Shaltout, A & Pelicon, P 2017, 'Molecular imaging of alkaloids in khat (Catha edulis) leaves with MeV-SIMS', Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms, vol. 404, pp. 140-145. https://doi.org/10.1016/j.nimb.2017.01.063

TY - JOUR

T1 - Molecular imaging of alkaloids in khat (Catha edulis) leaves with MeV-SIMS

AU - Jencic, Bostjan

AU - Jeromel, Luka

AU - Potocnik, Nina Ogrinc

AU - Vogel-Mikus, Katarina

AU - Vavpetic, Primoz

AU - Rupnik, Zdravko

AU - Bucar, Klemen

AU - Vencelj, Matjaz

AU - Kelemen, Mitja

AU - Matsuo, Jiro

AU - Kusakari, Masakazu

AU - Siketic, Zdravko

AU - Al-Jalali, Muhammad A.

AU - Shaltout, Abdallah

AU - Pelicon, Primoz

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Imaging Mass Spectroscopy (IMS) is a unique research tool providing localization and identification of a wide range of biomolecules as essential data to understand biochemical processes in living organisms. Secondary Ion Mass Spectrometry with high-energy heavy ions (MeV-SIMS) is emerging as a promising IMS technique for chemical imaging of biological tissue.We measured the molecular mass spatial distributions in leaves of khat (Catha edulis). Khat is a natural drug plant, native to eastern Africa and the Arabian Peninsula. In these countries, fresh leaves are being chewed by significant part of population. It was reported that 80% of the adult population in Yemen chew the khat leaves. The main stimulating effects of khat are induced by a monoamine alkaloid called cathinone. During leaf ageing, cathinone is further metabolised to cathine and norephedrine. Earlier studies identified the alkaloids in khat, however little is known on their spatial distribution, reflecting the biosynthesis and accumulation in the tissue.Chemical mapping of alkaloids on cross-sections of khat leaves by MeV-SIMS was done at JSI by a pulsed 5.8 MeV Cl-35(6+) beam, focused to a diameter of 15 mu m, using a linear time-of-flight (TOF) spectrometer with a mass resolution of 500. In addition, measurements of MeV-SIMS mass spectra were performed at Kyoto University by a continuous broad beam of 6 MeV Cu-63(4+) ions at an orthogonal TOF spectrometer with a high mass resolution of 11,000. Sections of leaves were analysed and mass spectra obtained at both MeV-SIMS setups were compared. Tissue-level distributions of detected alkaloids are presented and discussed. (C) 2017 Elsevier B.V. All rights reserved.

AB - Imaging Mass Spectroscopy (IMS) is a unique research tool providing localization and identification of a wide range of biomolecules as essential data to understand biochemical processes in living organisms. Secondary Ion Mass Spectrometry with high-energy heavy ions (MeV-SIMS) is emerging as a promising IMS technique for chemical imaging of biological tissue.We measured the molecular mass spatial distributions in leaves of khat (Catha edulis). Khat is a natural drug plant, native to eastern Africa and the Arabian Peninsula. In these countries, fresh leaves are being chewed by significant part of population. It was reported that 80% of the adult population in Yemen chew the khat leaves. The main stimulating effects of khat are induced by a monoamine alkaloid called cathinone. During leaf ageing, cathinone is further metabolised to cathine and norephedrine. Earlier studies identified the alkaloids in khat, however little is known on their spatial distribution, reflecting the biosynthesis and accumulation in the tissue.Chemical mapping of alkaloids on cross-sections of khat leaves by MeV-SIMS was done at JSI by a pulsed 5.8 MeV Cl-35(6+) beam, focused to a diameter of 15 mu m, using a linear time-of-flight (TOF) spectrometer with a mass resolution of 500. In addition, measurements of MeV-SIMS mass spectra were performed at Kyoto University by a continuous broad beam of 6 MeV Cu-63(4+) ions at an orthogonal TOF spectrometer with a high mass resolution of 11,000. Sections of leaves were analysed and mass spectra obtained at both MeV-SIMS setups were compared. Tissue-level distributions of detected alkaloids are presented and discussed. (C) 2017 Elsevier B.V. All rights reserved.

KW - Imaging Mass Spectroscopy

KW - MeV-SIMS

KW - Khat

KW - Nuclear microprobe

KW - MICRO-PIXE

U2 - 10.1016/j.nimb.2017.01.063

DO - 10.1016/j.nimb.2017.01.063

M3 - Article

SN - 0168-583X

VL - 404

SP - 140

EP - 145

JO - Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms

JF - Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms

T2 - 15th International Conference on Nuclear Microprobe Technology and Applications (ICNMTA) / 6th International Workshop on Proton Beam Writing

Y2 - 31 July 2016 through 5 August 2016

ER -

Molecular imaging of alkaloids in khat (Catha edulis) leaves with MeV-SIMS

Abstract

Keywords

Access to Document

Fingerprint

Cite this