Molecular Imaging in Oncology: Advanced Microscopy Techniques

Dimitrios Kapsokalyvas; Marc A M J van Zandvoort

doi:10.1007/978-3-030-42618-7_16

Molecular Imaging in Oncology: Advanced Microscopy Techniques

Dimitrios Kapsokalyvas
, Marc A M J van Zandvoort^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

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Abstract

Preclinical studies usually require high levels of morphological, functional, and biochemical information at subcellular resolution. This type of information cannot be obtained from clinical imaging techniques, such as MRI, PET/CT, or US. Luckily, many microscopy techniques exist that can offer this information, also for malignant tissues and therapeutic approaches. In this overview, we discuss the various advanced optical microscopy techniques and their applications in oncological research. After a short introduction in Sect. 16.1, we continue in Sect. 16.2 with a discussion on fluorescent labelling strategies, followed in Sect. 16.3 by an in-depth description of confocal, light-sheet, two-photon, and super-resolution microscopy. We end in Sect. 16.4 with a focus on the applications, specifically in oncology.

Original language	English
Title of host publication	Molecular Imaging in Oncology
Chapter	16
Pages	533-561
Number of pages	29
Volume	216
ISBN (Electronic)	9783030426187
DOIs	https://doi.org/10.1007/978-3-030-42618-7_16
Publication status	Published - 2020

Publication series

Series	Recent Results in Cancer Research
ISSN	0080-0015

Keywords

Humans
Medical Oncology
Microscopy/methods
Molecular Imaging
Neoplasms/pathology

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title = "Molecular Imaging in Oncology: Advanced Microscopy Techniques",

abstract = "Preclinical studies usually require high levels of morphological, functional, and biochemical information at subcellular resolution. This type of information cannot be obtained from clinical imaging techniques, such as MRI, PET/CT, or US. Luckily, many microscopy techniques exist that can offer this information, also for malignant tissues and therapeutic approaches. In this overview, we discuss the various advanced optical microscopy techniques and their applications in oncological research. After a short introduction in Sect. 16.1, we continue in Sect. 16.2 with a discussion on fluorescent labelling strategies, followed in Sect. 16.3 by an in-depth description of confocal, light-sheet, two-photon, and super-resolution microscopy. We end in Sect. 16.4 with a focus on the applications, specifically in oncology.",

keywords = "Humans, Medical Oncology, Microscopy/methods, Molecular Imaging, Neoplasms/pathology",

author = "Dimitrios Kapsokalyvas and \{van Zandvoort\}, \{Marc A M J\}",

note = "Publisher Copyright: {\textcopyright} 2020, Springer Nature Switzerland AG.",

year = "2020",

doi = "10.1007/978-3-030-42618-7\_16",

language = "English",

volume = "216",

series = "Recent Results in Cancer Research",

publisher = "Springer",

pages = "533--561",

booktitle = "Molecular Imaging in Oncology",

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T1 - Molecular Imaging in Oncology

T2 - Advanced Microscopy Techniques

AU - Kapsokalyvas, Dimitrios

AU - van Zandvoort, Marc A M J

PY - 2020

Y1 - 2020

N2 - Preclinical studies usually require high levels of morphological, functional, and biochemical information at subcellular resolution. This type of information cannot be obtained from clinical imaging techniques, such as MRI, PET/CT, or US. Luckily, many microscopy techniques exist that can offer this information, also for malignant tissues and therapeutic approaches. In this overview, we discuss the various advanced optical microscopy techniques and their applications in oncological research. After a short introduction in Sect. 16.1, we continue in Sect. 16.2 with a discussion on fluorescent labelling strategies, followed in Sect. 16.3 by an in-depth description of confocal, light-sheet, two-photon, and super-resolution microscopy. We end in Sect. 16.4 with a focus on the applications, specifically in oncology.

AB - Preclinical studies usually require high levels of morphological, functional, and biochemical information at subcellular resolution. This type of information cannot be obtained from clinical imaging techniques, such as MRI, PET/CT, or US. Luckily, many microscopy techniques exist that can offer this information, also for malignant tissues and therapeutic approaches. In this overview, we discuss the various advanced optical microscopy techniques and their applications in oncological research. After a short introduction in Sect. 16.1, we continue in Sect. 16.2 with a discussion on fluorescent labelling strategies, followed in Sect. 16.3 by an in-depth description of confocal, light-sheet, two-photon, and super-resolution microscopy. We end in Sect. 16.4 with a focus on the applications, specifically in oncology.

KW - Humans

KW - Medical Oncology

KW - Microscopy/methods

KW - Molecular Imaging

KW - Neoplasms/pathology

U2 - 10.1007/978-3-030-42618-7_16

DO - 10.1007/978-3-030-42618-7_16

M3 - Chapter

C2 - 32594398

VL - 216

T3 - Recent Results in Cancer Research

SP - 533

EP - 561

BT - Molecular Imaging in Oncology

ER -

Molecular Imaging in Oncology: Advanced Microscopy Techniques

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