Molecular Imaging in Oncology: Advanced Microscopy Techniques

Research output: Contribution to journalReview articlepeer-review

Abstract

Preclinical studies usually require high levels of morphological, functional, and biochemical information at subcellular resolution. This type of information cannot be obtained from clinical imaging techniques, such as MRI, PET/CT, or US. Luckily, many microscopy techniques exist that can offer this information, also for malignant tissues and therapeutic approaches. In this overview, we discuss the various advanced optical microscopy techniques and their applications in oncological research. After a short introduction in Sect. 16.1, we continue in Sect. 16.2 with a discussion on fluorescent labelling strategies, followed in Sect. 16.3 by an in-depth description of confocal, light-sheet, two-photon, and super-resolution microscopy. We end in Sect. 16.4 with a focus on the applications, specifically in oncology.

Original languageEnglish
Pages (from-to)533-561
Number of pages29
JournalRecent Results in Cancer Research
Volume216
DOIs
Publication statusPublished - 2020

Keywords

  • Humans
  • Medical Oncology
  • Microscopy/methods
  • Molecular Imaging
  • Neoplasms/pathology

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