Molecular Imaging in Oncology: Advanced Microscopy Techniques

Dimitrios Kapsokalyvas, Marc A M J van Zandvoort*

*Corresponding author for this work

Research output: Contribution to journal(Systematic) Review article peer-review


Preclinical studies usually require high levels of morphological, functional, and biochemical information at subcellular resolution. This type of information cannot be obtained from clinical imaging techniques, such as MRI, PET/CT, or US. Luckily, many microscopy techniques exist that can offer this information, also for malignant tissues and therapeutic approaches. In this overview, we discuss the various advanced optical microscopy techniques and their applications in oncological research. After a short introduction in Sect. 16.1, we continue in Sect. 16.2 with a discussion on fluorescent labelling strategies, followed in Sect. 16.3 by an in-depth description of confocal, light-sheet, two-photon, and super-resolution microscopy. We end in Sect. 16.4 with a focus on the applications, specifically in oncology.

Original languageEnglish
Pages (from-to)533-561
Number of pages29
JournalRecent Results in Cancer Research
Publication statusPublished - 2020


  • Humans
  • Medical Oncology
  • Microscopy/methods
  • Molecular Imaging
  • Neoplasms/pathology

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