Molecular adaptation in adipose tissue in response to overfeeding with a high-fat diet under sedentary conditions in South Asian and Caucasian men

Siti N. Wulan*, Freek G. Bouwman, Klaas R. Westerterp, Edwin C. M. Mariman, Guy Plasqui

*Corresponding author for this work

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Abstract

For the same BMI, South Asians have a higher body fat percentage than Caucasians. There might be differences in the fatty acid (FA) handling in adipose tissue when both ethnicities are exposed to high-fat overfeeding. The objective of the present study was to investigate the molecular adaptation in relation to FA metabolism in response to overfeeding with a high-fat diet (OHFD) in South Asian and Caucasian men. Ten South Asian men (BMI 18-29 kg/m(2)) and ten Caucasian men (BMI 22-33 kg/m(2)), matched for body fat percentage, aged 20-40 years were included. A weight-maintenance diet (30 % fat, 55 % carbohydrate and 15 % protein) was given for 3 d followed by 3 d of overfeeding (150 % energy requirement) with a high-fat diet (60 % fat, 25 % carbohydrate and 15 % protein) while staying in a respiration chamber. Before and after overfeeding, abdominal subcutaneous fat biopsies were taken. Proteins were isolated, analysed and quantified for short-chain 3-hydroxyacyl-CoA dehydrogenase (HADH), carnitine palmitoyl-transferase 1 alpha (CPT1a), adipose TAG lipase, perilipin A (PLINA), perilipin B, lipoprotein lipase and fatty acid binding protein 4 using Western blotting. OHFD decreased the HADH level (P <0 center dot 05) in Caucasians more than in Asians (P <0 center dot 05), but the baseline and after intervention HADH level was relatively higher in Caucasians. The level of CPT1a decreased in South Asians and increased in Caucasians (P <0 center dot 05). PLINA did not change with diet but the level was higher in South Asians (P <0 center dot 05). The observed differences in HADH and PLINA levels as well as in CPT1a response may be important for differences in the long-term regulation of energy (fat) metabolism in these populations.

Original languageEnglish
Article number0007114519001260
Pages (from-to)241-251
Number of pages11
JournalBritish Journal of Nutrition
Volume122
Issue number3
DOIs
Publication statusPublished - 14 Aug 2019

Keywords

  • Molecular adaptation
  • Overfeeding
  • High-fat diet
  • South Asian men
  • Caucasian men
  • ENERGY-EXPENDITURE
  • TRIGLYCERIDE LIPASE
  • BODY-COMPOSITION
  • OXIDATION
  • OBESITY
  • PERILIPIN
  • QUANTITATION
  • ASSOCIATION
  • CAPACITY
  • PATTERNS

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