Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep

Candice C. Snyder; Katherine B. Wolfe; Tate Gisslen; Christine L. Knox; Matthew W. Kemp; Boris W. Kramer; John P. Newnham; Alan H. Jobe; Suhas G. Kallapur

doi:10.1016/j.ajog.2013.02.018

Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep

Candice C. Snyder^*, Katherine B. Wolfe, Tate Gisslen, Christine L. Knox, Matthew W. Kemp, Boris W. Kramer, John P. Newnham, Alan H. Jobe, Suhas G. Kallapur

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

OBJECTIVE: Ureaplasma colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that Ureaplasma colonization of amniotic fluid would modulate chorioamnionitis induced by Escherichia coli lipopolysaccharide (LPS). STUDY DESIGN: Sheep received intraamniotic (IA) injections of media (control) or live Ureaplasma either 7 or 70 days before delivery. Another group received IA LPS 2 days before delivery. To test for interactions, U parvum-exposed animals were challenged with IA LPS, and delivered 2 days later. All animals were delivered preterm at 125 +/- 1 day of gestation. RESULTS: Both IA Ureaplasma and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of proinflammatory cytokines and myeloperoxidase in leukocytes, while Ureaplasma alone caused modest responses. Interestingly, 7-day but not 70-day Ureaplasma exposure significantly down-regulated LPS-induced proinflammatory cytokines and myeloperoxidase expression in the chorioamnion. CONCLUSION: Acute (7-day) U parvum exposure can suppress LPS-induced chorioamnionitis.

Original language	English
Article number	399.e1
Journal	American Journal of Obstetrics and Gynecology
Volume	208
Issue number	5
DOIs	https://doi.org/10.1016/j.ajog.2013.02.018
Publication status	Published - May 2013

Keywords

endotoxin tolerance
fetal adaptation
innate immunity
preterm labor

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10.1016/j.ajog.2013.02.018

Cite this

@article{caffc7941e204407b445adaee14659fa,

title = "Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep",

abstract = "OBJECTIVE: Ureaplasma colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that Ureaplasma colonization of amniotic fluid would modulate chorioamnionitis induced by Escherichia coli lipopolysaccharide (LPS). STUDY DESIGN: Sheep received intraamniotic (IA) injections of media (control) or live Ureaplasma either 7 or 70 days before delivery. Another group received IA LPS 2 days before delivery. To test for interactions, U parvum-exposed animals were challenged with IA LPS, and delivered 2 days later. All animals were delivered preterm at 125 +/- 1 day of gestation. RESULTS: Both IA Ureaplasma and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of proinflammatory cytokines and myeloperoxidase in leukocytes, while Ureaplasma alone caused modest responses. Interestingly, 7-day but not 70-day Ureaplasma exposure significantly down-regulated LPS-induced proinflammatory cytokines and myeloperoxidase expression in the chorioamnion. CONCLUSION: Acute (7-day) U parvum exposure can suppress LPS-induced chorioamnionitis.",

keywords = "endotoxin tolerance, fetal adaptation, innate immunity, preterm labor",

author = "Snyder, {Candice C.} and Wolfe, {Katherine B.} and Tate Gisslen and Knox, {Christine L.} and Kemp, {Matthew W.} and Kramer, {Boris W.} and Newnham, {John P.} and Jobe, {Alan H.} and Kallapur, {Suhas G.}",

year = "2013",

month = may,

doi = "10.1016/j.ajog.2013.02.018",

language = "English",

volume = "208",

journal = "American Journal of Obstetrics and Gynecology",

issn = "0002-9378",

publisher = "MOSBY-ELSEVIER",

number = "5",

}

TY - JOUR

T1 - Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep

AU - Snyder, Candice C.

AU - Wolfe, Katherine B.

AU - Gisslen, Tate

AU - Knox, Christine L.

AU - Kemp, Matthew W.

AU - Kramer, Boris W.

AU - Newnham, John P.

AU - Jobe, Alan H.

AU - Kallapur, Suhas G.

PY - 2013/5

Y1 - 2013/5

N2 - OBJECTIVE: Ureaplasma colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that Ureaplasma colonization of amniotic fluid would modulate chorioamnionitis induced by Escherichia coli lipopolysaccharide (LPS). STUDY DESIGN: Sheep received intraamniotic (IA) injections of media (control) or live Ureaplasma either 7 or 70 days before delivery. Another group received IA LPS 2 days before delivery. To test for interactions, U parvum-exposed animals were challenged with IA LPS, and delivered 2 days later. All animals were delivered preterm at 125 +/- 1 day of gestation. RESULTS: Both IA Ureaplasma and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of proinflammatory cytokines and myeloperoxidase in leukocytes, while Ureaplasma alone caused modest responses. Interestingly, 7-day but not 70-day Ureaplasma exposure significantly down-regulated LPS-induced proinflammatory cytokines and myeloperoxidase expression in the chorioamnion. CONCLUSION: Acute (7-day) U parvum exposure can suppress LPS-induced chorioamnionitis.

AB - OBJECTIVE: Ureaplasma colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that Ureaplasma colonization of amniotic fluid would modulate chorioamnionitis induced by Escherichia coli lipopolysaccharide (LPS). STUDY DESIGN: Sheep received intraamniotic (IA) injections of media (control) or live Ureaplasma either 7 or 70 days before delivery. Another group received IA LPS 2 days before delivery. To test for interactions, U parvum-exposed animals were challenged with IA LPS, and delivered 2 days later. All animals were delivered preterm at 125 +/- 1 day of gestation. RESULTS: Both IA Ureaplasma and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of proinflammatory cytokines and myeloperoxidase in leukocytes, while Ureaplasma alone caused modest responses. Interestingly, 7-day but not 70-day Ureaplasma exposure significantly down-regulated LPS-induced proinflammatory cytokines and myeloperoxidase expression in the chorioamnion. CONCLUSION: Acute (7-day) U parvum exposure can suppress LPS-induced chorioamnionitis.

KW - endotoxin tolerance

KW - fetal adaptation

KW - innate immunity

KW - preterm labor

U2 - 10.1016/j.ajog.2013.02.018

DO - 10.1016/j.ajog.2013.02.018

M3 - Article

C2 - 23410690

SN - 0002-9378

VL - 208

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

IS - 5

M1 - 399.e1

ER -