Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep

Candice C. Snyder*, Katherine B. Wolfe, Tate Gisslen, Christine L. Knox, Matthew W. Kemp, Boris W. Kramer, John P. Newnham, Alan H. Jobe, Suhas G. Kallapur

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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OBJECTIVE: Ureaplasma colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that Ureaplasma colonization of amniotic fluid would modulate chorioamnionitis induced by Escherichia coli lipopolysaccharide (LPS). STUDY DESIGN: Sheep received intraamniotic (IA) injections of media (control) or live Ureaplasma either 7 or 70 days before delivery. Another group received IA LPS 2 days before delivery. To test for interactions, U parvum-exposed animals were challenged with IA LPS, and delivered 2 days later. All animals were delivered preterm at 125 +/- 1 day of gestation. RESULTS: Both IA Ureaplasma and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of proinflammatory cytokines and myeloperoxidase in leukocytes, while Ureaplasma alone caused modest responses. Interestingly, 7-day but not 70-day Ureaplasma exposure significantly down-regulated LPS-induced proinflammatory cytokines and myeloperoxidase expression in the chorioamnion. CONCLUSION: Acute (7-day) U parvum exposure can suppress LPS-induced chorioamnionitis.
Original languageEnglish
Article number399.e1
JournalAmerican Journal of Obstetrics and Gynecology
Issue number5
Publication statusPublished - May 2013


  • endotoxin tolerance
  • fetal adaptation
  • innate immunity
  • preterm labor

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