Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

K.A. Demin, T.O. Kolesnikova, D.S. Galstyan, N.A. Krotova, N.P. Ilyin, K.A. Derzhavina, N.A. Levchenko, T. Strekalova, M.S. de Abreu, E.V. Petersen, M. Seredinskaya, Y.V. Cherneyko, Y.M. Kositsyn, D.V. Sorokin, K.N. Zabegalov, M.S. Mor, E.V. Efimova, A.V. Kalueff*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Web of Science)

Abstract

Long-term recurrent stress is a common cause of neuropsychiatric disorders. Animal models are widely used to study the pathogenesis of stress-related psychiatric disorders. The zebrafish (Danio rerio) is emerging as a powerful tool to study chronic stress and its mechanisms. Here, we developed a prolonged 11-week chronic unpredictable stress (PCUS) model in zebrafish to more fully mimic chronic stress in human populations. We also examined behavioral and neurochemical alterations in zebrafish, and attempted to modulate these states by 3-week treatment with an antidepressant fluoxetine, a neuroprotective omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA), a pro-inflammatory endotoxin lipopolysaccharide (LPS), and their combinations. Overall, PCUS induced severe anxiety and elevated norepinephrine levels, whereas fluoxetine (alone or combined with other agents) corrected most of these behavioral deficits. While EPA and LPS alone had little effects on the zebrafish PCUS-induced anxiety behavior, both fluoxetine (alone or in combination) and EPA restored norepinephrine levels, whereas LPS+EPA increased dopamine levels. As these data support the validity of PCUS as an effective tool to study stress-related pathologies in zebrafish, further research is needed into the ability of various conventional and novel treatments to modulate behavioral and neurochemical biomarkers of chronic stress in this model organism.
Original languageEnglish
Article number14289
Number of pages15
JournalScientific Reports
Volume11
Issue number1
DOIs
Publication statusPublished - 12 Jul 2021

Keywords

  • CHRONIC MILD STRESS
  • DEPRESSION-LIKE BEHAVIOR
  • PITUITARY-ADRENAL AXIS
  • PROINFLAMMATORY CYTOKINES
  • NEUROTRANSMITTER SYSTEMS
  • NEUROSCIENCE RESEARCH
  • DOCOSAHEXAENOIC ACID
  • DOUBLE-BLIND
  • DOPAMINE
  • ANXIETY

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