TY - JOUR
T1 - Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures
AU - Sacchetto, C.
AU - Vitiello, L.
AU - de Windt, L.J.
AU - Rampazzo, A.
AU - Calore, M.
N1 - Funding Information:
L.J.d.W. and M.C. acknowledge support from the Netherlands CardioVascular Research Initiative (CVON ARENA-PRIME). L.J.D.W. was further supported by grant 311549 from the European Research Council (ERC) and a VICI award 918-156-47 from The Netherlands Organisation for Scientific Research (NWO). AR acknowledges support from TRANSAC Strategic Research Grant CPDA133979/13, University of Padua, Italy.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - In the last decade, the generation of cardiac disease models based on human-induced pluripotent stem cells (hiPSCs) has become of common use, providing new opportunities to overcome the lack of appropriate cardiac models. Although much progress has been made toward the generation of hiPSC-derived cardiomyocytes (hiPS-CMs), several lines of evidence indicate that two-dimensional (2D) cell culturing presents significant limitations, including hiPS-CMs immaturity and the absence of interaction between different cell types and the extracellular matrix. More recently, new advances in bioengineering and co-culture systems have allowed the generation of three-dimensional (3D) constructs based on hiPSC-derived cells. Within these systems, biochemical and physical stimuli influence the maturation of hiPS-CMs, which can show structural and functional properties more similar to those present in adult cardiomyocytes. In this review, we describe the latest advances in 2D- and 3D-hiPSC technology for cardiac disease mechanisms investigation, drug development, and therapeutic studies.
AB - In the last decade, the generation of cardiac disease models based on human-induced pluripotent stem cells (hiPSCs) has become of common use, providing new opportunities to overcome the lack of appropriate cardiac models. Although much progress has been made toward the generation of hiPSC-derived cardiomyocytes (hiPS-CMs), several lines of evidence indicate that two-dimensional (2D) cell culturing presents significant limitations, including hiPS-CMs immaturity and the absence of interaction between different cell types and the extracellular matrix. More recently, new advances in bioengineering and co-culture systems have allowed the generation of three-dimensional (3D) constructs based on hiPSC-derived cells. Within these systems, biochemical and physical stimuli influence the maturation of hiPS-CMs, which can show structural and functional properties more similar to those present in adult cardiomyocytes. In this review, we describe the latest advances in 2D- and 3D-hiPSC technology for cardiac disease mechanisms investigation, drug development, and therapeutic studies.
KW - 3d cardiac models
KW - cardiac disease modeling
KW - cardiac microtissues
KW - cardiomyopathy
KW - endothelial-cells
KW - engineered heart tissue
KW - engineered human myocardium
KW - functional maturation
KW - human induced pluripotent stem cells
KW - improve recovery
KW - metabolic maturation
KW - pluripotent stem-cell
KW - qt syndrome
KW - tissue
KW - QT SYNDROME
KW - CARDIAC MICROTISSUES
KW - METABOLIC MATURATION
KW - FUNCTIONAL MATURATION
KW - ENDOTHELIAL-CELLS
KW - ENGINEERED HUMAN MYOCARDIUM
KW - TISSUE
KW - CARDIOMYOPATHY
KW - 3D cardiac models
KW - PLURIPOTENT STEM-CELL
KW - IMPROVE RECOVERY
U2 - 10.3390/ijms21093404
DO - 10.3390/ijms21093404
M3 - (Systematic) Review article
C2 - 32403456
SN - 1661-6596
VL - 21
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 9
M1 - 3404
ER -