Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures

C. Sacchetto, L. Vitiello, L.J. de Windt, A. Rampazzo*, M. Calore*

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

21 Citations (Web of Science)

Abstract

In the last decade, the generation of cardiac disease models based on human-induced pluripotent stem cells (hiPSCs) has become of common use, providing new opportunities to overcome the lack of appropriate cardiac models. Although much progress has been made toward the generation of hiPSC-derived cardiomyocytes (hiPS-CMs), several lines of evidence indicate that two-dimensional (2D) cell culturing presents significant limitations, including hiPS-CMs immaturity and the absence of interaction between different cell types and the extracellular matrix. More recently, new advances in bioengineering and co-culture systems have allowed the generation of three-dimensional (3D) constructs based on hiPSC-derived cells. Within these systems, biochemical and physical stimuli influence the maturation of hiPS-CMs, which can show structural and functional properties more similar to those present in adult cardiomyocytes. In this review, we describe the latest advances in 2D- and 3D-hiPSC technology for cardiac disease mechanisms investigation, drug development, and therapeutic studies.
Original languageEnglish
Article number3404
Number of pages32
JournalInternational Journal of Molecular Sciences
Volume21
Issue number9
DOIs
Publication statusPublished - 1 May 2020

Keywords

  • 3d cardiac models
  • cardiac disease modeling
  • cardiac microtissues
  • cardiomyopathy
  • endothelial-cells
  • engineered heart tissue
  • engineered human myocardium
  • functional maturation
  • human induced pluripotent stem cells
  • improve recovery
  • metabolic maturation
  • pluripotent stem-cell
  • qt syndrome
  • tissue
  • QT SYNDROME
  • CARDIAC MICROTISSUES
  • METABOLIC MATURATION
  • FUNCTIONAL MATURATION
  • ENDOTHELIAL-CELLS
  • ENGINEERED HUMAN MYOCARDIUM
  • TISSUE
  • CARDIOMYOPATHY
  • 3D cardiac models
  • PLURIPOTENT STEM-CELL
  • IMPROVE RECOVERY

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