Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Peter Moller; Amaya Azqueta; Elisa Boutet-Robinet; Gudrun Koppen; Stefano Bonassi; Mirta Milic; Goran Gajski; Solange Costa; Joao Paulo Teixeira; Cristiana Costa Pereira; Maria Dusinska; Roger Godschalk; Gunnar Brunborg; Kristine B. Gutzkow; Lisa Giovannelli; Marcus S. Cooke; Elke Richling; Blanca Laffon; Vanessa Valdiglesias; Nursen Basaran; Cristian Del Bo'; Bojana Zegura; Matjaz Novak; Helga Stopper; Pavel Vodicka; Sona Vodenkova; Vanessa Moraes de Andrade; Monika Sramkova; Alena Gabelova; Andrew Collins; Sabine A. S. Langie

doi:10.1038/s41596-020-0398-1

Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Peter Moller^*, Amaya Azqueta, Elisa Boutet-Robinet, Gudrun Koppen, Stefano Bonassi, Mirta Milic, Goran Gajski, Solange Costa, Joao Paulo Teixeira, Cristiana Costa Pereira, Maria Dusinska, Roger Godschalk, Gunnar Brunborg, Kristine B. Gutzkow, Lisa Giovannelli, Marcus S. Cooke, Elke Richling, Blanca Laffon, Vanessa Valdiglesias, Nursen BasaranCristian Del Bo', Bojana Zegura, Matjaz Novak, Helga Stopper, Pavel Vodicka, Sona Vodenkova, Vanessa Moraes de Andrade, Monika Sramkova, Alena Gabelova, Andrew Collins, Sabine A. S. Langie

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.

Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).

Original language	English
Pages (from-to)	3817-3826
Number of pages	10
Journal	Nature Protocols
Volume	15
Issue number	12
Early online date	26 Oct 2020
DOIs	https://doi.org/10.1038/s41596-020-0398-1
Publication status	Published - Dec 2020

Keywords

INTER-LABORATORY VARIATION
DNA-DAMAGE
ANIMAL-MODELS
CELLS
REPAIR
TIME
MIGRATION
OXIDATION
WHALES
BASE

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Cite this

Moller, P., Azqueta, A., Boutet-Robinet, E., Koppen, G., Bonassi, S., Milic, M., Gajski, G., Costa, S., Teixeira, J. P., Costa Pereira, C., Dusinska, M., Godschalk, R., Brunborg, G., Gutzkow, K. B., Giovannelli, L., Cooke, M. S., Richling, E., Laffon, B., Valdiglesias, V., ... Langie, S. A. S. (2020). Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. Nature Protocols, 15(12), 3817-3826. https://doi.org/10.1038/s41596-020-0398-1

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abstract = "The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).",

keywords = "INTER-LABORATORY VARIATION, DNA-DAMAGE, ANIMAL-MODELS, CELLS, REPAIR, TIME, MIGRATION, OXIDATION, WHALES, BASE",

author = "Peter Moller and Amaya Azqueta and Elisa Boutet-Robinet and Gudrun Koppen and Stefano Bonassi and Mirta Milic and Goran Gajski and Solange Costa and Teixeira, {Joao Paulo} and {Costa Pereira}, Cristiana and Maria Dusinska and Roger Godschalk and Gunnar Brunborg and Gutzkow, {Kristine B.} and Lisa Giovannelli and Cooke, {Marcus S.} and Elke Richling and Blanca Laffon and Vanessa Valdiglesias and Nursen Basaran and {Del Bo'}, Cristian and Bojana Zegura and Matjaz Novak and Helga Stopper and Pavel Vodicka and Sona Vodenkova and {de Andrade}, {Vanessa Moraes} and Monika Sramkova and Alena Gabelova and Andrew Collins and Langie, {Sabine A. S.}",

note = "Publisher Copyright: {\textcopyright} 2020, The Author(s), under exclusive licence to Springer Nature Limited.",

year = "2020",

month = dec,

doi = "10.1038/s41596-020-0398-1",

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Moller, P, Azqueta, A, Boutet-Robinet, E, Koppen, G, Bonassi, S, Milic, M, Gajski, G, Costa, S, Teixeira, JP, Costa Pereira, C, Dusinska, M, Godschalk, R, Brunborg, G, Gutzkow, KB, Giovannelli, L, Cooke, MS, Richling, E, Laffon, B, Valdiglesias, V, Basaran, N, Del Bo', C, Zegura, B, Novak, M, Stopper, H, Vodicka, P, Vodenkova, S, de Andrade, VM, Sramkova, M, Gabelova, A, Collins, A & Langie, SAS 2020, 'Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results', Nature Protocols, vol. 15, no. 12, pp. 3817-3826. https://doi.org/10.1038/s41596-020-0398-1

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T2 - recommendations for describing comet assay procedures and results

AU - Moller, Peter

AU - Azqueta, Amaya

AU - Boutet-Robinet, Elisa

AU - Koppen, Gudrun

AU - Bonassi, Stefano

AU - Milic, Mirta

AU - Gajski, Goran

AU - Costa, Solange

AU - Teixeira, Joao Paulo

AU - Costa Pereira, Cristiana

AU - Dusinska, Maria

AU - Godschalk, Roger

AU - Brunborg, Gunnar

AU - Gutzkow, Kristine B.

AU - Giovannelli, Lisa

AU - Cooke, Marcus S.

AU - Richling, Elke

AU - Laffon, Blanca

AU - Valdiglesias, Vanessa

AU - Basaran, Nursen

AU - Del Bo', Cristian

AU - Zegura, Bojana

AU - Novak, Matjaz

AU - Stopper, Helga

AU - Vodicka, Pavel

AU - Vodenkova, Sona

AU - de Andrade, Vanessa Moraes

AU - Sramkova, Monika

AU - Gabelova, Alena

AU - Collins, Andrew

AU - Langie, Sabine A. S.

PY - 2020/12

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N2 - The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).

AB - The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).

KW - INTER-LABORATORY VARIATION

KW - DNA-DAMAGE

KW - ANIMAL-MODELS

KW - CELLS

KW - REPAIR

KW - TIME

KW - MIGRATION

KW - OXIDATION

KW - WHALES

KW - BASE

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DO - 10.1038/s41596-020-0398-1

M3 - Article

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SN - 1754-2189

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JO - Nature Protocols

JF - Nature Protocols

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