TY - JOUR
T1 - Minimum Information for Reporting on the Comet Assay (MIRCA)
T2 - recommendations for describing comet assay procedures and results
AU - Moller, Peter
AU - Azqueta, Amaya
AU - Boutet-Robinet, Elisa
AU - Koppen, Gudrun
AU - Bonassi, Stefano
AU - Milic, Mirta
AU - Gajski, Goran
AU - Costa, Solange
AU - Teixeira, Joao Paulo
AU - Costa Pereira, Cristiana
AU - Dusinska, Maria
AU - Godschalk, Roger
AU - Brunborg, Gunnar
AU - Gutzkow, Kristine B.
AU - Giovannelli, Lisa
AU - Cooke, Marcus S.
AU - Richling, Elke
AU - Laffon, Blanca
AU - Valdiglesias, Vanessa
AU - Basaran, Nursen
AU - Del Bo', Cristian
AU - Zegura, Bojana
AU - Novak, Matjaz
AU - Stopper, Helga
AU - Vodicka, Pavel
AU - Vodenkova, Sona
AU - de Andrade, Vanessa Moraes
AU - Sramkova, Monika
AU - Gabelova, Alena
AU - Collins, Andrew
AU - Langie, Sabine A. S.
PY - 2020/12
Y1 - 2020/12
N2 - The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).
AB - The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).
KW - INTER-LABORATORY VARIATION
KW - DNA-DAMAGE
KW - ANIMAL-MODELS
KW - CELLS
KW - REPAIR
KW - TIME
KW - MIGRATION
KW - OXIDATION
KW - WHALES
KW - BASE
U2 - 10.1038/s41596-020-0398-1
DO - 10.1038/s41596-020-0398-1
M3 - Article
C2 - 33106678
VL - 15
SP - 3817
EP - 3826
JO - Nature Protocols
JF - Nature Protocols
SN - 1754-2189
IS - 12
ER -